[从鱼藤中分离鉴定卤虫致死活性成分]
[Isolation and identification of brine shrimp lethal activities from Derris trifoliata].
作者信息
Jiang Cheng, Xiong Ben-qiang, Qiu Xi-min, Liu Sheng-zi, Zhang Si, Yin Hao
机构信息
Department of Pharmacy, Medical College, Hunan Normal University, Changsha 410006, China.
出版信息
Zhong Yao Cai. 2012 May;35(5):719-23.
OBJECTIVE
To study the brine shrimp lethal activities of the roots and stems from Derris trifoliata.
METHODS
The biological activity of the athyl acetate extract was tested by the 2nd-instar of Artemia franciscana by the method of Silica gel column chromatography, Sephadex LH-20 column chromatography, Semipreparative HPLC, and their structures were elucidated by spectral analysis.
RESULTS
Eleven compounds were isolated from the athyl acetate extract, and LD50 s of 12a-hydroxyrotenone, tephrosin, rotenone and deguelin were 0.365, 0.236, 0.060 and 0.734 microg/mL, respectively.
CONCLUSION
The results of bioassay show that 12a-hydroxyrotenone, tephrosin,rotenone and deguelin have strong brine shrimp lethal activity.
目的
研究鱼藤根和茎对卤虫的致死活性。
方法
采用硅胶柱色谱、葡聚糖凝胶LH-20柱色谱、半制备高效液相色谱法对乙酸乙酯提取物的生物活性进行测试，用光谱分析方法对其结构进行鉴定。
结果
从乙酸乙酯提取物中分离得到11个化合物，12α-羟基鱼藤酮、灰叶素、鱼藤酮和脱氢鱼藤素的半数致死剂量分别为0.365、0.236、0.060和0.734μg/mL。
结论
生物活性测定结果表明，12α-羟基鱼藤酮、灰叶素、鱼藤酮和脱氢鱼藤素具有较强的卤虫致死活性。
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