Jiao Wei, Liu Fei, Li Hai-lan, Wu Huan-xiu, Lan Jiao, Xiao Rui-ping, Qin Lin, Jiang Bin
The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China.
Fa Yi Xue Za Zhi. 2012 Oct;28(5):355-8.
To investigate the genetic polymorphisms of 16 Y-STR loci and to evaluate the forensic application in Miao, Yao and Dong nationalities of Guangxi population.
Genotypes of Y-STR loci were tested in a total of 253 healthy unrelated individuals (67 Miao people, 99 Yao people, 87 Dong people) using AmpFlSTR Yfiler PCR amplification kit. Allele frequencies and population genetics parameters of the 16 Y-STR loci were statistically analyzed. The allele frequencies were compared among the three nationalities.
Most alleles were detected at locus DYS385 while fewest alleles were detected at locus DYS437 among the three nationalities. GD values were ranged from 0.2619 (DYS438) to 0.9417 (DYS385) for Miao nationality, 0.3170 (DYS391) to 0.955 9 (DYS385) for Yao nationality and 0.305 3 (DYS391) to 0.943 3 (DYS385) for Dong nationality, respectively. There were no statistically significant differences detected (P>0.05) at loci of DYS391 and DYS438 among the three nationalities.
The 16 Y-STR loci can be applied to practices and basic research of forensic genetics in the three main nationalities of Guangxi population.
研究16个Y染色体短串联重复序列（Y-STR）基因座的遗传多态性，并评估其在广西苗族、瑶族和侗族人群中的法医学应用价值。
采用AmpFlSTR Yfiler PCR扩增试剂盒，对广西253名健康无关个体（苗族67人、瑶族99人、侗族87人）进行Y-STR基因座分型检测。对16个Y-STR基因座的等位基因频率和群体遗传学参数进行统计学分析，并比较三个民族间的等位基因频率。
三个民族中，DYS385基因座检测到的等位基因数最多，DYS437基因座检测到的等位基因数最少。苗族的基因多样性（GD）值范围为0.2619（DYS438）至0.9417（DYS385），瑶族为0.3170（DYS391）至0.9559（DYS385），侗族为0.3053（DYS391）至0.9433（DYS385）。三个民族在DYS391和DYS438基因座上差异无统计学意义（P>0.05）。
16个Y-STR基因座可应用于广西三个主要民族人群的法医学遗传学实践及基础研究。
