Li Qian, Gao Su-Qing, Li Heng-Cong, Wang Da-Ming, Zeng Jian-Qiang, Deng Zhi-Hui
Shenzhen Blood Center, Shenzhen 518035, Guangdong Province, China.
Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2008 Jun;16(3):694-8.
The aim of this study was to investigate the genetic polymorphism of Y-chromosome specific short tandem repeat (Y-STR) loci in Zhuang ethnic group of China. Nine Y-STR loci were amplified by single multiplex and the PCR products were detected by using ABI Prism(TM) 3100 DNA Sequencer. The allele frequencies and haplotype frequencies at 9 Y-STR loci were determined in a total of 85 unrelated male individuals from Zhuang ethnic group of China. The results indicated that in the 85 unrelated male individuals, except for the DYS426 locus with a low GD value, the GD values for other 8 Y-STR loci ranged from 0.4387 to 0.8129. A total of 70 haplotypes at 9 Y-STR loci were found, the haplotype diversity was 0.9926. It is concluded that the haplotype polymorphism of 9 Y-STR loci are highly polymorphic in Zhuang ethnic group and also significantly different from our previous reported data of unrelated male individnals in southern Chinese Han population.
本研究旨在调查中国壮族人群Y染色体特异性短串联重复序列(Y-STR)位点的基因多态性。采用单管多重PCR扩增9个Y-STR位点,并使用ABI Prism™ 3100 DNA测序仪检测PCR产物。在总共85名来自中国壮族的无关男性个体中确定了9个Y-STR位点的等位基因频率和单倍型频率。结果表明,在这85名无关男性个体中,除DYS426位点杂合度值较低外,其他8个Y-STR位点的杂合度值在0.4387至0.8129之间。在9个Y-STR位点共发现70种单倍型,单倍型多样性为0.9926。结论是,9个Y-STR位点的单倍型多态性在壮族人群中具有高度多态性,且与我们之前报道的中国南方汉族无关男性个体的数据也有显著差异。