Department of Applied Biological Science, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
J Org Chem. 2013 Jan 18;78(2):320-7. doi: 10.1021/jo302127d. Epub 2012 Dec 24.
A soluble tag-assisted liquid-phase peptide synthesis was successfully established based on simple hydrophobic benzyl alcohols, which can be easily prepared from naturally abundant materials. Excellent precipitation yields can be obtained at each step, combining the best properties of solid-phase and liquid-phase techniques. This approach can also be applied efficiently to fragment couplings, allowing chemical synthesis of several bioactive peptides.
基于简单的疏水性苄醇，成功建立了一种可溶性标签辅助的液相肽合成方法，这些苄醇可以很容易地从天然丰富的材料中制备得到。在每一步都可以得到极好的沉淀产率，结合了固相和液相技术的最佳特性。这种方法也可以有效地应用于片段偶联，允许对几种生物活性肽进行化学合成。
