Wang Meng, Xing Jun, Sun Yu-Tang, Guo Ling-Xiang, Lin Bao-Ping, Yang Hong
School of Chemistry and Chemical Engineering, Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, Jiangsu Key Laboratory for Science and Application of Molecular Ferroelectrics, Southeast University Nanjing 211189 China
RSC Adv. 2018 Mar 27;8(22):11843-11849. doi: 10.1039/c7ra13320k. eCollection 2018 Mar 26.
Chemical incorporation of enzymes onto polymeric materials has recently attracted intense scientific attention. Cross-linked polysiloxane gels as a typical super-hydrophobic support, are a good candidate for supporting enzymes in low-water organic medium to efficiently catalyze peptide synthesis because the hydrophobic polysiloxane matrix can prevent water from attacking the acyl-enzyme intermediate, which is beneficial for the shift in equilibrium to peptide formation. In this work, we develop a facile strategy to photoimmobilize olefin-functionalized chymotrypsin onto cross-linked polysiloxane gels UV-initiated thiol-ene click chemistry. The impacts of water addition amount, heat-treatment and recyclability of the immobilized chymotrypsin influencing the peptide synthesis efficiency are investigated. Compared with the native chymotrypsin, polysiloxane-immobilized chymotrypsin showed advantageous catalytic activity, higher thermal stability and superior recyclability.
酶在聚合物材料上的化学结合近来引起了科学界的广泛关注。交联聚硅氧烷凝胶作为一种典型的超疏水载体，是在低水有机介质中支持酶以有效催化肽合成的良好候选材料，因为疏水性聚硅氧烷基质可以防止水攻击酰基酶中间体，这有利于平衡向肽形成方向移动。在这项工作中，我们开发了一种简便的策略，通过紫外光引发的硫醇-烯点击化学将烯烃功能化的胰凝乳蛋白酶光固定在交联聚硅氧烷凝胶上。研究了固定化胰凝乳蛋白酶的加水量、热处理和可回收性对肽合成效率的影响。与天然胰凝乳蛋白酶相比，聚硅氧烷固定化的胰凝乳蛋白酶表现出有利的催化活性、更高的热稳定性和优异的可回收性。
