White R P, Leffler C W, Bada H S
Department of Pharmacology, University of Tennessee Medical Center, Memphis 38163.
Am J Med Sci. 1990 Apr;299(4):230-5. doi: 10.1097/00000441-199004000-00003.
The cerebrospinal fluid (CSF) of 11 premature infants suffering from posthemorrhagic hydrocephalus was examined by radioimmunoassay for prostaglandin (PG) E2, PGF2 alpha, PGD2, 6-keto PGF1 alpha, thromboxane B2 (TxB2) and peptidoleukotrienes (LTC4/LTD4). The LTs were detected in the CSF of more of these patients (70%) than any of the other eicosanoids, and usually in the highest concentration. Among the 11 posthemorrhagic patients CSF eicosanoid levels were highest when determined soon after injury. Moreover, the variety of eicosanoids present, as well as concentrations, in these infants decreased with time. The types of eicosanoids most evident in the CSF of patients who required shunting were TxB2 and LTs, being present together in 5 of 6 (83%) of these infants. In contrast, 1 of 5 (20%) of the patients who did not require this neurosurgical intervention contained both TxB2 and LTs, the remaining having only one or neither eicosanoid. The highest average concentration for each eicosanoid studied was (pg/ml): PGE2, 628; PGF2 alpha, 985; PGD2, 1410; 6-keto PGF1 alpha, 544; TxB2, 486 and LTs, 1229. This study is the first to demonstrate that the CSF of preterm infants may contain a wide variety of eicosanoids and indicates that these lipids are a manifestation of neurological assault.
采用放射免疫分析法对11例出血后脑积水早产儿的脑脊液（CSF）进行检测，测定其中前列腺素（PG）E2、PGF2α、PGD2、6 - 酮 - PGF1α、血栓素B2（TxB2）和肽白三烯（LTC4/LTD4）的含量。与其他类二十烷酸相比，更多此类患者（70%）的脑脊液中可检测到白三烯，且其浓度通常最高。在11例出血后患者中，受伤后不久测定时脑脊液类二十烷酸水平最高。此外，这些婴儿脑脊液中存在的类二十烷酸种类及其浓度均随时间下降。在需要分流的患者脑脊液中最明显的类二十烷酸类型是TxB2和白三烯，6例此类婴儿中有5例（83%）同时存在这两种物质。相比之下，5例不需要这种神经外科干预的患者中有1例（20%）同时含有TxB2和白三烯，其余患者仅含有一种类二十烷酸或两种都没有。所研究的每种类二十烷酸的最高平均浓度（pg/ml）分别为：PGE2，628；PGF2α，985；PGD2，1410；6 - 酮 - PGF1α，544；TxB2，486；白三烯，1229。本研究首次证明早产儿脑脊液中可能含有多种类二十烷酸，并表明这些脂质是神经损伤的一种表现。
