Yang Y C, Hearing P, Rundell K
J Virol. 1979 Oct;32(1):147-54. doi: 10.1128/JVI.32.1.147-154.1979.
Immunoprecipitates of extracts of simian virus 40-infected permissive monkey kidney cells contained two proteins with molecular weights of 56,000 and 32,000 (52K and 32K) in addition to the known viral early gene products. Immunoprecipitates of cells infected with the 0.54-0.59 deletion mutants that lack the viral 17K gene product did not contain thhe 56K and 32K proteins. The additional proteins appeared in immunoprecipitates of deletion mutant extracts if unlabeled extracts of wild-type-infected cells were added before addition of antiserum. The proteins can also be identified in uninfected cells by co-precipitation with unlabeled viral proteins. Thus, it appears that the 56K and 32K proteins are cellular products that associate with the viral proteins, the 17K in particular, and are indirectly immunoprecipitated by anti-tumor serum.
除了已知的病毒早期基因产物外，感染猿猴病毒40的允许性猴肾细胞提取物的免疫沉淀物中还含有两种分子量分别为56,000和32,000（52K和32K）的蛋白质。感染了缺乏病毒17K基因产物的0.54 - 0.59缺失突变体的细胞的免疫沉淀物中不含有56K和32K蛋白质。如果在加入抗血清之前加入野生型感染细胞的未标记提取物，那么在缺失突变体提取物的免疫沉淀物中就会出现这些额外的蛋白质。通过与未标记的病毒蛋白共沉淀，也可以在未感染的细胞中鉴定出这些蛋白质。因此，看来56K和32K蛋白质是与病毒蛋白，特别是17K蛋白相关的细胞产物，并被抗肿瘤血清间接免疫沉淀。
