Koncki R, Mohr G J, Wolfbeis O S
Institute of Organic Chemistry, Karl-Franzens University, Graz, Austria.
Biosens Bioelectron. 1995 Fall;10(8):653-9. doi: 10.1016/0956-5663(95)96955-x.
A new, absorbance-based enzymatic biosensor membrane for determination of urea is described. A lipophilic, fully LED- and diode laser-compatible pH sensitive dye was incorporated into a plasticized, carboxylated poly(vinyl chloride) membrane and served as the optical transducer of the sensor. Urease was covalently linked to the surface of the pH bulk optode membrane to form a very thin cover. The resulting biosensor membrane allows rapid determination of urea over the 0.3 to 100 mM range. The reproducibility, stability, and effects of pH and buffer concentration on the response of sensor are reported. The preparation of the pH transducer and the immobilization of the enzyme are simple and may easily be adopted to other biosensor types.
描述了一种用于测定尿素的新型基于吸光度的酶促生物传感器膜。一种亲脂性、完全与发光二极管和二极管激光器兼容的pH敏感染料被掺入增塑的羧化聚氯乙烯膜中，并用作传感器的光学换能器。脲酶与pH整体光极膜表面共价连接，形成非常薄的覆盖层。所得的生物传感器膜能够在0.3至100 mM范围内快速测定尿素。报告了该传感器的重现性、稳定性以及pH和缓冲液浓度对其响应的影响。pH换能器的制备和酶的固定化方法简单，并且可以很容易地应用于其他类型的生物传感器。
