Universidade Federal do Paraná, Departamento de Bioquímica e Biologia Molecular, CP 19046, CEP 81531-980, Curitiba, PR, Brazil.
Carbohydr Polym. 2013 Jan 30;92(1):192-9. doi: 10.1016/j.carbpol.2012.08.113. Epub 2012 Sep 7.
Endosperms from seeds of different subfamilies of Leguminosae were submitted to sequential aqueous and alkaline aqueous extractions. The extractions from species belonging to the Mimosoideae and Faboideae subfamilies yielded galactomannans with constant Man:Gal ratios, whereas the extractions from Caesalpinioideae seeds gave rise to galactomannans with increasing values of the Man:Gal ratio. The presence of a family of galactomannans within the same species may be a trait found only in Caesalpinioideae subfamily. The final insoluble residues that were obtained after the removal of galactomannans from the Caesalpinioideae and Faboideae subfamilies are composed of pure mannans and do not contain cellulose, while those from the Mimosoideae subfamily are composed of cellulose. A mannan was isolated from the unripe endosperm of Caesalpinia pulcherrima, suggesting no developmental relationship between galactomannan and mannan. These results are consistent with the presence of a distinctive cell wall pattern in the endosperms of Leguminosae species.
不同豆科亚科种子的胚乳分别经过连续的水提和碱性水提。来自含羞草亚科和蝶形花亚科的物种的提取物得到了甘露糖:半乳糖比例恒定的半乳甘露聚糖，而来自苏木亚科种子的提取物则得到了甘露糖:半乳糖比例不断增加的半乳甘露聚糖。同一物种中存在半乳甘露聚糖家族可能是仅在苏木亚科中发现的特征。从苏木亚科和蝶形花亚科中去除半乳甘露聚糖后获得的最终不溶性残渣由纯甘露聚糖组成，不含有纤维素，而来自含羞草亚科的残渣则由纤维素组成。从美丽决明未成熟胚乳中分离得到甘露聚糖，表明半乳甘露聚糖和甘露聚糖之间没有发育关系。这些结果与豆科物种胚乳中存在独特的细胞壁模式一致。
