BASF SE, Carl-Bosch-Straße 38, 67056, Ludwigshafen, Germany.
BASF Enzymes LLC, 3550 John Hopkins Court, San Diego, CA, 92121, USA.
Sci Rep. 2024 Jan 10;14(1):1037. doi: 10.1038/s41598-024-51494-w.
For farm animals the supplementation of exogenous enzymes, like ß-mannanase, to soybean-based diets is beneficial to improve feed digestibility. In order to unravel the effect of ß-mannanase on soybean meal's cell structure, a novel imaging concept was developed which allows visualizing the spatial activity pattern of ß-mannanase with high sensitivity by fluorescence microscopy before any visible degradation of the cellular structure occurs. It is based on fluorescence labeling of newly formed reducing ends of ß-mannanase-hydrolyzed polysaccharides after the native reducing ends of all polysaccharides present were chemically reduced. It was revealed that ß-mannanase is not only active at the cell wall but also at previously unknown sites, like the middle lamella and, most prominently, at an intracellular matrix enclosing the protein storage vacuoles. Based on these findings it can be hypothesized that the evaluated ß-mannanase can degrade the enclosing matrix of encapsulated proteins and the cell wall structure and thereby improves efficiency of feed utilization.
对于农场动物来说，在以大豆为基础的饲料中补充外源酶，如β-甘露聚糖酶，有利于提高饲料的消化率。为了揭示β-甘露聚糖酶对大豆粉细胞结构的影响，开发了一种新的成像概念，该概念允许通过荧光显微镜在细胞结构发生任何可见降解之前，以高灵敏度可视化β-甘露聚糖酶的空间活性模式。它基于β-甘露聚糖酶水解多糖的新形成还原末端的荧光标记，而所有存在的多糖的天然还原末端被化学还原。结果表明，β-甘露聚糖酶不仅在细胞壁上具有活性，而且在以前未知的部位，如中层和最突出的是包围蛋白储存液泡的细胞内基质上具有活性。基于这些发现，可以假设所评估的β-甘露聚糖酶可以降解包裹蛋白质的基质和细胞壁结构，从而提高饲料利用效率。
