阿尔及利亚撒哈拉地区药用植物传统应用的药理学研究：从种子中提取的水溶性多糖的抗炎和抗高血糖活性的体外研究
Pharmacological Investigations in Traditional Utilization of Medik. in Saharan Algeria: In Vitro Study of Anti-Inflammatory and Antihyperglycemic Activities of Water-Soluble Polysaccharides Extracted from the Seeds.
作者信息
Chakou Fatma Zohra, Boual Zakaria, Hadj Mohamed Didi Ould El, Belkhalfa Hakim, Bachari Khaldoun, El Alaoui-Talibi Zainab, El Modafar Cherkaoui, Hadjkacem Farah, Fendri Imen, Abdelkafi Slim, Traïkia Mounir, Cerf Didier Le, Dubessay Pascal, Delattre Cédric, Pierre Guillaume, Michaud Philippe
机构信息
Laboratory for the Protection of Ecosystems in Arid and Semi-Arid Zones, Kasdi Merbah-University, Ouargla 30000, Algeria.
Institut Pascal, Université Clermont Auvergne, CNRS, Clermont Auvergne INP, 63000 Clermont-Ferrand, France.
出版信息
Plants (Basel). 2021 Dec 3;10(12):2658. doi: 10.3390/plants10122658.
The anti-inflammatory and antihyperglycemic effects of polysaccharides extracted from Medik. seeds, spontaneous shrub collected in Southern of Algerian Sahara were investigated. Their water extraction followed by alcoholic precipitation was conducted to obtain two water-soluble polysaccharides extracts (WSPAM1 and WSPAM2). They were characterized using Fourier transform infrared, H/C Nuclear Magnetic Resonance, Gas Chromatography-Mass Spectrometry and Size Exclusion Chromatography coupled with Multi-Angle Light Scattering. The capacity of those fractions to inhibit α-amylase activity and thermally induced Bovine Serum Albumin denaturation were also investigated. WSPAM1 and WSPAM2 were galactomannans with a mannose/galactose ratio of 2.2 and 2.4, respectively. The SEC-MALLS analysis revealed that WSPAM1 had a molecular weight of 1.4 × 10 Da. The investigations highlighted antinflammatory and antihyperglycemic effects in a dose-dependant manner of WSPAM1 and WSPAM2.
对从阿尔及利亚撒哈拉沙漠南部采集的自生灌木Medik.种子中提取的多糖的抗炎和降血糖作用进行了研究。通过水提取后乙醇沉淀的方法，获得了两种水溶性多糖提取物（WSPAM1和WSPAM2）。使用傅里叶变换红外光谱、H/C核磁共振、气相色谱 - 质谱联用和尺寸排阻色谱结合多角度光散射对它们进行了表征。还研究了这些组分抑制α - 淀粉酶活性和热诱导牛血清白蛋白变性的能力。WSPAM1和WSPAM2是半乳甘露聚糖，甘露糖/半乳糖比分别为2.2和2.4。尺寸排阻色谱 - 多角度光散射分析表明，WSPAM1的分子量为1.4×10 Da。研究突出了WSPAM1和WSPAM2以剂量依赖方式的抗炎和降血糖作用。
Zotero 插件