Department of Food Technology, Kongu Engineering College, Perundurai, Erode 638052, TN, India.
Carbohydr Polym. 2013 Jan 30;92(1):604-11. doi: 10.1016/j.carbpol.2012.09.020. Epub 2012 Sep 16.
In this study, ultrasound assisted extraction (UAE) conditions on the yield of polysaccharide from corn silk were studied using three factors, three level Box-Behnken response surface design. Process parameters, which affect the efficiency of UAE such as extraction temperature (40-60 °C), time (10-30 min) and solid-liquid ratio (1:10-1:30 g/ml) were investigated. The results showed that, the extraction conditions have significant effects on extraction yield of polysaccharide. The obtained experimental data were fitted to a second-order polynomial equation using multiple regression analysis with high coefficient of determination value (R(2)) of 0.994. An optimization study using Derringer's desired function methodology was performed and the optimal conditions based on both individual and combinations of all independent variables (extraction temperature of 56 °C, time of 17 min and solid-liquid ratio of 1:20 g/ml) were determined with maximum polysaccharide yield of 6.06%, which was confirmed through validation experiments.
本研究采用三因素三水平 Box-Behnken 响应面设计，研究了超声辅助提取（UAE）条件对玉米须多糖产率的影响。考察了提取温度（40-60°C）、时间（10-30 分钟）和固液比（1:10-1:30 g/ml）等影响 UAE 效率的工艺参数。结果表明，提取条件对多糖提取率有显著影响。用多元回归分析对实验数据进行拟合，得到了二次多项式方程，具有较高的决定系数（R(2)）为 0.994。采用 Derringer 的期望函数法进行优化研究，确定了基于各独立变量（提取温度为 56°C，时间为 17 分钟，固液比为 1:20 g/ml）的最佳条件，多糖得率最高为 6.06%，通过验证实验得到了证实。
