Zhu Guilan, Cheng Xiaozhong, Fu Zijing, Zhang Zhilan, Tong Qunyi
1Department of Life Science, Hefei Normal University, Hefei, 230061 China.
2School of Food Science and Technology, Jiangnan University, Wuxi, 214122 China.
3 Biotech. 2020 Feb;10(2):70. doi: 10.1007/s13205-019-2047-3. Epub 2020 Jan 25.
A two-stage culture (with controlled sucrose concentrations and temperatures) of for gellan gum production has been previously investigated. Herein, the mechanism of a two-stage culture favoring gellan gum overproduction was revealed by analysing the cell-membrane permeability and the proteomics for gellan gum biosynthesis. The two-stage culture, resulted in 79.8% increased content of unsaturated fatty acids, and 3.95% increased ratio of unsaturated to saturated fatty acids in the cell membrane. Moreover, cell membrane permeability increased and thus further enhanced gellan gum biosynthesis. Proteomic analysis results indicated that 13 identified protein spots were involved in energy generation, glycogen biosynthesis, and glycolysis. These findings revealed that two-stage culture impellel carbon flux flow toward gellan gum biosynthesis.
先前已经研究了用于结冷胶生产的两阶段培养（控制蔗糖浓度和温度）。在此，通过分析细胞膜通透性和结冷胶生物合成的蛋白质组学，揭示了两阶段培养有利于结冷胶过量生产的机制。两阶段培养导致细胞膜中不饱和脂肪酸含量增加79.8%，不饱和脂肪酸与饱和脂肪酸的比例增加3.95%。此外，细胞膜通透性增加，从而进一步增强了结冷胶的生物合成。蛋白质组学分析结果表明，13个已鉴定的蛋白质斑点参与能量产生、糖原生物合成和糖酵解。这些发现表明，两阶段培养促使碳通量流向结冷胶生物合成。
