ICMR Virus Unit, ID & BG Hospital, GB-4, First Floor, 57 Dr Suresh C Banerjee Road, Beliaghata, Kolkata 700 010, India.
Microbiol Res. 2013 May 6;168(4):238-44. doi: 10.1016/j.micres.2012.11.002. Epub 2012 Dec 5.
The aim of this study was to evaluate the antiviral potential of methanolic extract (ME) of Achyranthes aspera, an Indian folk medicine and one of its pure compound oleanolic acid (OA) against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2). The ME possessed weak anti-herpes virus activity (EC50 64.4μg/ml for HSV-1 and 72.8μg/ml for HSV-2). While OA exhibited potent antiherpesvirus activity against both HSV-1 (EC50 6.8μg/ml) and HSV-2 (EC50 7.8μg/ml). The time response study revealed that the antiviral activity of ME and OA is highest at 2-6h post infection. The infected and drug-treated peritoneal macrophage at specific time showed increased level of pro-inflammatory cytokines (IL6 and IL12). Further, the PCR of DNA from infected cultures treated with ME and OA, at various time intervals, failed to show amplification at 48-72h, similar to that of HSV infected cells treated with acyclovir, indicating that the ME and OA probably inhibit the early stage of multiplication (post infection of 2-6h). Thus, our study demonstrated that ME and OA have good anti-HSV activity, with SI values of 12, suggesting the potential use of this plant.
本研究旨在评估印度民间药物牛膝草的甲醇提取物 (ME) 及其纯化合物齐墩果酸 (OA) 对单纯疱疹病毒 1 型 (HSV-1) 和 2 型 (HSV-2) 的抗病毒潜力。ME 对单纯疱疹病毒具有较弱的抗病毒活性（对 HSV-1 的 EC50 为 64.4μg/ml，对 HSV-2 的 EC50 为 72.8μg/ml）。而 OA 对 HSV-1（EC50 为 6.8μg/ml）和 HSV-2（EC50 为 7.8μg/ml）均表现出强大的抗疱疹病毒活性。时间反应研究表明，感染后 2-6 小时，ME 和 OA 的抗病毒活性最高。在特定时间点，感染和药物处理的腹腔巨噬细胞显示出促炎细胞因子（IL6 和 IL12）水平升高。此外，用 ME 和 OA 处理的感染培养物的 DNA 的 PCR 在不同时间间隔内未能在 48-72 小时显示扩增，类似于用阿昔洛韦处理的感染细胞，表明 ME 和 OA 可能抑制病毒的早期增殖阶段（感染后 2-6 小时）。因此，我们的研究表明 ME 和 OA 具有良好的抗 HSV 活性，SI 值为 12，表明该植物具有潜在的应用价值。
