Department of Pharmacology, Toxicology and Legal and Forensic Medicine, Veterinary Faculty, University of Córdoba, Córdoba 14071, Spain.
Ecotoxicol Environ Saf. 2013 Feb;88:155-62. doi: 10.1016/j.ecoenv.2012.11.010. Epub 2012 Dec 6.
There is great social concern about the risk involved from exposure to BPA as an endocrine disrupter in humans, as well as the possible repercussion of this chemical on the environment. In this study, the short-term effects of BPA at a gonadal level were assessed by means of different biomarkers in a model animal organism in vogue, the zebrafish (Danio rerio). For this purpose, 60 female zebrafish aged 16 weeks were used. These were exposed for 14 days in aquariums (following OECD Directive no.204) to BPA concentrations of 1, 10, 100 and 1000 μg/L, in addition to a control batch. After the exposure period, the zebrafish were sacrificed and samples taken for a histopathological study by light and electron microscopy and morphometric analysis. During the fourteen days of exposure, water samples were taken from the aquariums to analyze the BPA levels. The BPA concentration in the fish and the water was determined by LC-MS/MS. The gonads of the zebrafish exposed to the BPA had a normal external appearance and there were no variations in their size or body weight. An accumulation of BPA was produced in the zebrafish tissues, and this increased as the BPA concentration to which the fish were exposed did. In the histopathological and morphometric studies, multiple alterations were observed in the zebrafish ovaries, particularly highlighting the vacuolization of the follicular cytoplasm, a great degeneration of all the cell components, and an important increase in the percentage of atretic follicles as from concentrations of 100 and 1000 μg/L of BPA, verified by morphometry. These data indicate that morphological endpoints are sufficiently sensitive to individuate early effects of environmental concentration of BPA on gonads after two weeks of exposure.
人们非常关注 BPA 作为一种内分泌干扰物对人类的风险，以及这种化学物质对环境可能产生的影响。在这项研究中，通过在流行的模式动物斑马鱼（Danio rerio）中使用不同的生物标志物，评估了 BPA 在性腺水平上的短期影响。为此，使用了 60 只 16 周龄的雌性斑马鱼。这些斑马鱼在水族箱中暴露了 14 天（遵循 OECD 指令 204），暴露于 1、10、100 和 1000μg/L 的 BPA 浓度中，此外还有一个对照组。暴露期结束后，处死斑马鱼并采集样本进行光镜和电子显微镜组织学研究和形态计量学分析。在 14 天的暴露期间，从水族箱中采集水样以分析 BPA 水平。通过 LC-MS/MS 测定鱼和水中的 BPA 浓度。暴露于 BPA 的斑马鱼的性腺外观正常，大小或体重没有变化。BPA 在斑马鱼组织中积累，并且随着鱼暴露于 BPA 的浓度增加而增加。在组织学和形态计量学研究中，观察到斑马鱼卵巢发生了多种改变，特别是滤泡细胞质的空泡化、所有细胞成分的严重退化以及从 100 和 1000μg/L 的 BPA 浓度开始，闭锁卵泡的百分比显著增加，通过形态计量学验证。这些数据表明，形态学终点足够敏感，可以在两周的暴露后识别出环境浓度的 BPA 对性腺的早期影响。
