Dipartimento Scienze della Vita e dell'Ambiente, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy.
Department of Biological Sciences, University of Calgary, Calgary, AB T2N 1N4, Canada.
Int J Mol Sci. 2021 Aug 27;22(17):9314. doi: 10.3390/ijms22179314.
Although the use of bisphenol A (BPA) has been banned in a number of countries, its presence in the environment still creates health issues both for humans and wildlife. So far, BPA toxicity has been largely investigated on different biological processes, from reproduction to development, immune system, and metabolism. In zebrafish, , previous studies revealed the ability of environmentally relevant concentrations of this contaminant to significantly impair fertility via epigenetic modification. In addition, several studies demonstrated the ability of different probiotic strains to improve organism health. This study provides information on the role of the probiotic mixture SLAb51 to counteract adverse BPA effects on reproduction. A 28-day trial was set up with different experimental groups: BPA, exposed to 10 µg/L BPA; P, receiving a dietary supplementation of SLAb51 at a final concentration of 10 CFU/g; BPA+P exposed to 10 µg/L BPA and receiving SLAb51 at a final concentration of 10 CFU/g and a C group. Since oocyte growth and maturation represent key aspects for fertility in females, studies were performed on isolated class III (vitellogenic) and IV (in maturation) follicles and liver, with emphasis on the modulation of the different vitellogenin isoforms. In males, key signals regulating spermatogenesis were investigated. Results demonstrated that in fish exposed to the combination of BPA and probiotic, most of the transcripts were closer to C or P levels, supporting the hypothesis of SLAb51 to antagonize BPA toxicity. This study represents the first evidence related to the use of SLAb51 to improve reproduction and open new fields of investigation regarding its use to reduce endocrine disrupting compound impacts on health.
尽管一些国家已经禁止使用双酚 A（BPA），但其在环境中的存在仍然对人类和野生动物的健康造成了问题。到目前为止，BPA 的毒性已经在许多不同的生物过程中得到了广泛研究，包括生殖、发育、免疫系统和新陈代谢。在斑马鱼中，[1]，先前的研究表明，这种污染物在环境相关浓度下通过表观遗传修饰显著损害生育能力的能力。此外，一些研究表明，不同益生菌菌株能够改善机体健康。本研究提供了关于益生菌混合物 SLAb51 抵抗 BPA 对生殖系统的不良影响的作用的信息。进行了为期 28 天的试验，包括不同的实验组：BPA，暴露于 10µg/L 的 BPA；P，摄入最终浓度为 10CFU/g 的 SLAb51 饮食补充剂；BPA+P，暴露于 10µg/L 的 BPA 并摄入最终浓度为 10CFU/g 的 SLAb51 和 C 组。由于卵母细胞的生长和成熟是女性生育能力的关键方面，因此在分离的 III 类（卵黄生成）和 IV 类（成熟）滤泡和肝脏中进行了研究，重点研究了不同卵黄蛋白原同工型的调节。在雄性中，研究了调节精子发生的关键信号。结果表明，在暴露于 BPA 和益生菌混合物的鱼类中，大多数转录本更接近 C 或 P 水平，这支持了 SLAb51 拮抗 BPA 毒性的假设。本研究代表了使用 SLAb51 改善生殖的第一个证据，并为其在减少内分泌干扰化合物对健康影响方面的应用开辟了新的研究领域。
