Departamento de Farmacología, Toxicología y Medicina Legal y Forense, Facultad de Veterinaria, Universidad de Córdoba, Campus de Rabanales, Edificio Darwin, 14071 Córdoba, Spain.
Departamento de Bioquímica y Biología Molecular, Campus de Excelencia Internacional Agroalimentario CeiA3, Universidad de Córdoba, Campus de Rabanales, Edificio Severo Ochoa, 14071 Córdoba, Spain.
Food Chem Toxicol. 2018 Feb;112:19-25. doi: 10.1016/j.fct.2017.12.026. Epub 2017 Dec 16.
Given the importance of bisphenol A (BPA) as a xenoestrogen and its potential effects on human and animal health, we evaluated BPA exposure's short-term effects on follicular development, yolk protein vitellogenin (VTG) production and aromatase expression in female zebrafish. Histological modifications were observed along with increased presence of atretic follicles. Whole-body VTG concentration increased with the dose of BPA exposure. In contrast, expression of Cyp19a mRNA in the ovaries of BPA-exposed fish exhibited an apparent non-monotonic response curve, marked by downregulation at 1 μg/L BPA, upregulation at 10 μg/L BPA, and a return to downregulation at 100 μg/L BPA and higher doses. Ovaries only exhibited significant increases in follicular atresia and VTG concentration after exposure to 100 μg/L BPA and higher doses. Ovarian histopathology, aromatase Cyp19a transcript levels and whole-body VTG protein abundance may be good biomarkers for early detection of environmental BPA exposure.
鉴于双酚 A（BPA）作为一种外源性雌激素的重要性及其对人类和动物健康的潜在影响，我们评估了 BPA 暴露对雌性斑马鱼卵泡发育、卵黄蛋白 vitellogenin（VTG）产生和芳香酶表达的短期影响。观察到组织学改变，同时存在更多的闭锁卵泡。全身 VTG 浓度随 BPA 暴露剂量的增加而增加。相比之下，暴露于 BPA 的鱼类卵巢中 Cyp19a mRNA 的表达表现出明显的非单调响应曲线，在 1μg/L BPA 时下调，在 10μg/L BPA 时上调，在 100μg/L BPA 及更高剂量时再次下调。只有在暴露于 100μg/L BPA 及更高剂量后，卵巢才会出现明显的卵泡闭锁和 VTG 浓度增加。卵巢组织病理学、芳香酶 Cyp19a 转录水平和全身 VTG 蛋白丰度可能是早期检测环境 BPA 暴露的良好生物标志物。
