Parums D V, Brown D L, Mitchinson M J
Department of Pathology, University of Cambridge, England.
Arch Pathol Lab Med. 1990 Apr;114(4):383-7.
The incidence of serum antibodies to human low-density lipoprotein, to oxidized low-density lipoprotein, and to ceroid extracted from human atheroma was assessed in 100 subjects using an adaptation of the enzyme-linked immunosorbent assay technique. Patients with chronic periaortitis, subclinical chronic periaortitis, and ischemic heart disease, and "elderly control" individuals were compared with young, healthy adults. Provided that precautions were taken to prevent oxidation of the low-density lipoprotein during the assay, antibodies were not found to native human low-density lipoprotein. Antibodies to oxidized low-density lipoprotein or ceroid, usually both, were detected in all 20 patients with clinical chronic periaortitis, in 17 of 20 patients with subclinical chronic periaortitis, in 12 of 20 patients with ischemic heart disease, and in 10 of 20 elderly control subjects. Binding inhibition studies showed cross-reactions between oxidized low-density lipoprotein and ceroid. Western blotting after sodium dodecyl sulfate polyacrylamide gel electrophoresis showed that in some patients with clinical chronic periaortitis, these antibodies were directed against breakdown products of apolipoprotein B that resulted from oxidation of low-density lipoprotein. Antibodies to oxidized low-density lipoprotein or ceroid were not detected in healthy young adults. These findings show that chronic periaortitis is accompanied by autoallergy to ceroid, which is probably at least partly composed of low-density lipoprotein oxidized within the human atherosclerotic plaque, and that a number of middle-aged and elderly people without chronic periaortitis also have such antibodies.
采用改良的酶联免疫吸附测定技术，对100名受试者检测了其血清中针对人低密度脂蛋白、氧化型低密度脂蛋白以及从人动脉粥样硬化斑块中提取的类蜡质的抗体发生率。将患有慢性主动脉周炎、亚临床慢性主动脉周炎和缺血性心脏病的患者，以及“老年对照”个体与年轻健康成年人进行了比较。只要在检测过程中采取预防措施防止低密度脂蛋白氧化，就未发现针对天然人低密度脂蛋白的抗体。在所有20例临床慢性主动脉周炎患者、20例亚临床慢性主动脉周炎患者中的17例、20例缺血性心脏病患者中的12例以及20例老年对照受试者中的10例中，均检测到了针对氧化型低密度脂蛋白或类蜡质（通常两者都有）的抗体。结合抑制研究表明氧化型低密度脂蛋白和类蜡质之间存在交叉反应。十二烷基硫酸钠聚丙烯酰胺凝胶电泳后的蛋白质印迹分析表明，在一些临床慢性主动脉周炎患者中，这些抗体针对的是由低密度脂蛋白氧化产生的载脂蛋白B的降解产物。在健康年轻成年人中未检测到针对氧化型低密度脂蛋白或类蜡质的抗体。这些发现表明，慢性主动脉周炎伴有对类蜡质的自身过敏反应，类蜡质可能至少部分由人动脉粥样硬化斑块内氧化的低密度脂蛋白组成，并且一些没有慢性主动脉周炎的中老年人也有此类抗体。
