一种用于检测鸡体内鸡毒支原体和滑液支原体血清抗体的抗生物素蛋白-生物素增强斑点免疫结合试验。

An avidin-biotin enhanced dot-immunobinding assay for the detection of Mycoplasma gallisepticum and M. synoviae serum antibodies in chickens.

作者信息

Cummins D R, Reynolds D L, Rhoades K R

机构信息

National Veterinary Services Laboratories, U.S. Department of Agriculture, Ames, Iowa 50010.

出版信息

Avian Dis. 1990 Jan-Mar;34(1):36-43.

PMID:2322229

Abstract

A dot-immunobinding assay was enhanced by the incorporation of avidin and biotin reagents into the test system (DAB assay). This assay was used to detect serum antibodies to Mycoplasma gallisepticum (MG) and M. synoviae (MS) from chickens. Serum samples were tested by rapid serum plate (RSP), hemagglutination-inhibition (HI), and DAB assay methods. These results were compared. The DAB assay was at least 20 times more sensitive in detecting antibodies for MS and at least 75 times more sensitive in detecting antibodies for MG than the HI test. The DAB assay was as specific as the HI test. The DAB assay was also more sensitive and specific than the RSP test. Some cross-reactions occurred when low dilutions of high-titer sera were used in the DAB assay. Parameters for determining negative, suspicious, and positive samples were established. The DAB assay for MG and MS may have several applications, including use as a screening test and a confirmatory test.

摘要

通过在检测系统（DAB检测）中加入抗生物素蛋白和生物素试剂，增强了斑点免疫结合检测法。该检测法用于检测鸡血清中针对鸡毒支原体（MG）和滑液支原体（MS）的抗体。血清样本通过快速血清平板（RSP）、血凝抑制（HI）和DAB检测法进行检测。对这些结果进行了比较。与HI检测相比，DAB检测在检测MS抗体方面至少敏感20倍，在检测MG抗体方面至少敏感75倍。DAB检测与HI检测一样具有特异性。DAB检测也比RSP检测更敏感和特异。当在DAB检测中使用高滴度血清的低稀释度时，会出现一些交叉反应。确定了阴性、可疑和阳性样本的判定参数。针对MG和MS的DAB检测可能有多种应用，包括用作筛查试验和确证试验。