New biotin-conjugated antisera for quantitation of Mycoplasma gallisepticum-specific immunoglobulin A in chicken.
作者信息
Barbour E K, Newman J A, Sivanandan V, Sasipreeyajan J
机构信息
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Minnesota, St. Paul 55108.
出版信息
Avian Dis. 1988 Jul-Sep;32(3):416-20.
PMID:3196259
Abstract
The biotinylation of goat anti-alpha-chains of chicken immunoglobulin A (IgA), suitable for use in an avidin-biotin enzyme-linked immunosorbent assay, is described. The optimum conditions for the use of the developed conjugate in determining local and systemic IgA specific to Mycoplasma gallisepticum in chickens were established.
摘要
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