Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, Minneapolis, Minnesota, USA.
Nat Chem Biol. 2013 Feb;9(2):81-3. doi: 10.1038/nchembio.1138. Epub 2012 Dec 9.
Engineering functional protein scaffolds capable of carrying out chemical catalysis is a major challenge in enzyme design. Starting from a noncatalytic protein scaffold, we recently generated a new RNA ligase by in vitro directed evolution. This artificial enzyme lost its original fold and adopted an entirely new structure with substantially enhanced conformational dynamics, demonstrating that a primordial fold with suitable flexibility is sufficient to carry out enzymatic function.
工程化具有化学催化功能的蛋白质支架是酶设计的主要挑战。我们最近从非催化蛋白支架出发，通过体外定向进化生成了一种新的 RNA 连接酶。这种人工酶失去了原有的折叠结构，采用了一种全新的结构，构象动力学大大增强，这表明具有适当灵活性的原始折叠足以执行酶功能。
