Laboratoire d'Ecologie-Ecotoxicologie, Faculté des Sciences, EA4689 Interaction Animal-Environnement, Université de Reims Champagne-Ardenne, Moulin de Housse, BP 1039-51687, Reims Cedex 2, France.
Fish Physiol Biochem. 2013 Aug;39(4):957-66. doi: 10.1007/s10695-012-9754-6. Epub 2012 Dec 7.
Antioxidative defenses and more especially catalasic activity (CAT) are studied in a large range of scientific research thematics. In environmental sciences, the problematic of oxidative stress is of great interest as pollutants can induce perturbations of redox homeostasis. Consequently, changes in antioxidative defenses levels in fish tissues and particularly in liver are used as potential biomarkers of pollution. In most studies, the CAT was assayed by following during 5 min the consumption of H2O2 in cytosolic buffered extracts at 240 nm (UV-method). This study proposed a development of this method in the visible, using permanganate and a 525-nm detection, which was more accurate, sensitive, and rapid. Moreover, the hepatic CAT of six different fish species [a cyclidae (Nimbochromis linni), 3 cyprinidae (Brachydanio rerio, Rutilus rutilus, Cyprinus carpio), an anguillidae (Anguilla anguilla), and a percidae (Perca fluviatilus)] was evaluated with the two protocols (UV- and KMnO4-method). The results but also the thermal optimum of the reaction and the interest of CAT as biomarker in ecotoxicology were discussed.
抗氧化防御系统，特别是过氧化氢酶（CAT）的活性，在广泛的科学研究主题中都有研究。在环境科学中，氧化应激问题引起了极大的关注，因为污染物会引起氧化还原平衡的紊乱。因此，鱼类组织中抗氧化防御水平的变化，特别是肝脏中的变化，被用作污染的潜在生物标志物。在大多数研究中，CAT 是通过在 240nm（UV 法）下在细胞溶质缓冲提取物中测定 H2O2 的消耗来测定的。本研究提出了一种在可见光谱中使用高锰酸盐和 525nm 检测的方法，该方法更准确、更灵敏、更快速。此外，还使用两种方法（UV 法和 KMnO4 法）评估了六种不同鱼类（一种丽鱼科（Nimbochromis linni）、三种鲤科（Brachydanio rerio、Rutilus rutilus、Cyprinus carpio）、一种鳗鲡科（Anguilla anguilla）和一种鮨科（Perca fluviatilus））的肝 CAT。讨论了结果以及反应的最适温度和 CAT 作为生态毒理学生物标志物的意义。
