Paris-Palacios Séverine, Biagianti-Risbourg Sylvie, Vernet Guy
Université de Reims Champagne-Ardenne, UFR Sciences Exactes et Naturelles, Metalliques, Reims, France.
Biomarkers. 2003 Mar-Apr;8(2):128-41. doi: 10.1080/1354750021000050511.
A variety of stresses, hormones, glucocorticoids and cytokines are known to induce metallothioneins (MTs) in animals. The aim of this study was to investigate the effects of chemical stress induced by the dicarboximide fungicide procymidone on hepatic structure, MT content and antioxidative defences (catalase and glutathione reductase activities and glutathione content) in the common fish Rutilus rutilus. Catalase and glutathione reductase activities remained stable throughout the experiment. Four days of exposure to 0.2 or 0.4 mg l(-1) of procymidone induced an obvious increase in liver MT content, perturbation of metal MT contents, and an increase in hepatic glutathione content. After 14 days' exposure, obvious and large structural alterations of the hepatic parenchyma occurred simultaneously with a decrease in MT and glutathione content. These events were interpreted as degeneration of the liver. Fish exposed for 14 days to procymidone and then placed for 14 days in clean water showed nearly complete decontamination of the liver, but MT concentrations remained high. The toxicological significance of these events is discussed.
已知多种应激、激素、糖皮质激素和细胞因子可诱导动物体内的金属硫蛋白（MTs）。本研究的目的是调查二甲酰亚胺类杀菌剂腐霉利诱导的化学应激对普通鱼类赤睛鱼肝脏结构、MT含量和抗氧化防御（过氧化氢酶和谷胱甘肽还原酶活性以及谷胱甘肽含量）的影响。在整个实验过程中，过氧化氢酶和谷胱甘肽还原酶活性保持稳定。暴露于0.2或0.4 mg l(-1)的腐霉利四天会导致肝脏MT含量明显增加、金属MT含量紊乱以及肝脏谷胱甘肽含量增加。暴露14天后，肝实质出现明显且较大的结构改变，同时MT和谷胱甘肽含量下降。这些事件被解释为肝脏退化。暴露于腐霉利14天然后置于清洁水中14天的鱼类显示肝脏几乎完全净化，但MT浓度仍然很高。讨论了这些事件的毒理学意义。
