• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用特异性和广泛氘化的脂质和 ¹³C-²H 旋转回波双共振固态 NMR 对肽和蛋白质进行残基特异性膜定位。

Residue-specific membrane location of peptides and proteins using specifically and extensively deuterated lipids and ¹³C-²H rotational-echo double-resonance solid-state NMR.

机构信息

Department of Chemistry, Michigan State University, 578 S. Shaw Lane, East Lansing, MI 48824, USA.

出版信息

J Biomol NMR. 2013 Jan;55(1):11-7. doi: 10.1007/s10858-012-9692-8. Epub 2012 Dec 8.

DOI:10.1007/s10858-012-9692-8
PMID:23225071
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3557618/
Abstract

Residue-specific location of peptides in the hydrophobic core of membranes was examined using (13)C-(2)H REDOR and samples in which the lipids were selectively deuterated. The transmembrane topology of the KALP peptide was validated with this approach with substantial dephasing observed for deuteration in the bilayer center and reduced or no dephasing for deuteration closer to the headgroups. Insertion of β sheet HIV and helical and β sheet influenza virus fusion peptides into the hydrophobic core of the membrane was validated in samples with extensively deuterated lipids.

摘要

使用 (13)C-(2)H REDOR 并对脂质进行选择性氘化的方法，研究了肽在膜疏水核心中的残基特异性定位。通过这种方法验证了 KALP 肽的跨膜拓扑结构，在双层中心的氘化观察到大量去相位，而靠近头部的氘化则减少或没有去相位。在脂质广泛氘化的样品中，验证了 HIV 的β 片层和螺旋及β 片层流感病毒融合肽插入膜疏水核心。

相似文献

1
Residue-specific membrane location of peptides and proteins using specifically and extensively deuterated lipids and ¹³C-²H rotational-echo double-resonance solid-state NMR.使用特异性和广泛氘化的脂质和 ¹³C-²H 旋转回波双共振固态 NMR 对肽和蛋白质进行残基特异性膜定位。
J Biomol NMR. 2013 Jan;55(1):11-7. doi: 10.1007/s10858-012-9692-8. Epub 2012 Dec 8.
2
Multiple locations of peptides in the hydrocarbon core of gel-phase membranes revealed by peptide (13)C to lipid (2)H rotational-echo double-resonance solid-state nuclear magnetic resonance.通过肽（¹³C）到脂质（²H）旋转回波双共振固态核磁共振揭示凝胶相膜烃核中肽的多个位置。
Biochemistry. 2015 Jan 27;54(3):677-84. doi: 10.1021/bi501211x. Epub 2015 Jan 9.
3
Solid-state nuclear magnetic resonance measurements of HIV fusion peptide 13CO to lipid 31P proximities support similar partially inserted membrane locations of the α helical and β sheet peptide structures.固态核磁共振测量 HIV 融合肽 13CO 与脂质 31P 的接近程度，支持 α 螺旋和 β 片肽结构的类似部分插入膜位置。
J Phys Chem A. 2013 Oct 3;117(39):9848-59. doi: 10.1021/jp312845w. Epub 2013 Feb 28.
4
REDOR solid-state NMR as a probe of the membrane locations of membrane-associated peptides and proteins.旋转回波双共振固态核磁共振技术作为一种探测膜相关肽和蛋白质在膜中位置的手段。
J Magn Reson. 2015 Apr;253:154-65. doi: 10.1016/j.jmr.2014.12.020.
5
H nuclear magnetic resonance spectroscopy supports larger amplitude fast motion and interference with lipid chain ordering for membrane that contains β sheet human immunodeficiency virus gp41 fusion peptide or helical hairpin influenza virus hemagglutinin fusion peptide at fusogenic pH.H 核磁共振光谱支持较大振幅的快速运动，并干扰脂质链排列，这种干扰对于在融合 pH 值下含有β 片层人类免疫缺陷病毒 gp41 融合肽或螺旋发夹状流感病毒血凝素融合肽的膜是存在的。
Biochim Biophys Acta Biomembr. 2020 Oct 1;1862(10):183404. doi: 10.1016/j.bbamem.2020.183404. Epub 2020 Jun 23.
6
Membrane topology of a 14-mer model amphipathic peptide: a solid-state NMR spectroscopy study.一种14聚体模型两亲性肽的膜拓扑结构：一项固态核磁共振波谱研究。
Biochemistry. 2007 Jun 5;46(22):6597-606. doi: 10.1021/bi0620151. Epub 2007 May 8.
7
REDOR NMR on a hydrophobic peptide in oriented membranes.在取向膜中对疏水肽进行旋转回波双共振核磁共振（REDOR NMR）研究。
J Magn Reson. 2000 Dec;147(2):366-70. doi: 10.1006/jmre.2000.2187.
8
High resolution ¹³C-detected solid-state NMR spectroscopy of a deuterated protein.高分辨率¹³C 检测的氘代蛋白质固态 NMR 光谱学。
J Biomol NMR. 2010 Oct;48(2):103-11. doi: 10.1007/s10858-010-9442-8. Epub 2010 Aug 29.
9
Effects of deuteration on solid-state NMR spectra of single peptide crystals and oriented protein samples.氘化对单肽晶体和定向蛋白样品固态 NMR 谱的影响。
J Magn Reson. 2019 Dec;309:106613. doi: 10.1016/j.jmr.2019.106613. Epub 2019 Sep 24.
10
Limits of Resolution and Sensitivity of Proton Detected MAS Solid-State NMR Experiments at 111 kHz in Deuterated and Protonated Proteins.在氘代和质子化蛋白质中，111 kHz 下质子检测 MAS 固态 NMR 实验的分辨率和灵敏度的限制。
Sci Rep. 2017 Aug 7;7(1):7444. doi: 10.1038/s41598-017-07253-1.

引用本文的文献

1
Three Decades of REDOR in Protein Science: A Solid-State NMR Technique for Distance Measurement and Spectral Editing.三十年的 REDOR 在蛋白质科学中的应用：一种用于距离测量和光谱编辑的固态 NMR 技术。
Int J Mol Sci. 2023 Sep 4;24(17):13637. doi: 10.3390/ijms241713637.
2
H nuclear magnetic resonance spectroscopy supports larger amplitude fast motion and interference with lipid chain ordering for membrane that contains β sheet human immunodeficiency virus gp41 fusion peptide or helical hairpin influenza virus hemagglutinin fusion peptide at fusogenic pH.H 核磁共振光谱支持较大振幅的快速运动，并干扰脂质链排列，这种干扰对于在融合 pH 值下含有β 片层人类免疫缺陷病毒 gp41 融合肽或螺旋发夹状流感病毒血凝素融合肽的膜是存在的。
Biochim Biophys Acta Biomembr. 2020 Oct 1;1862(10):183404. doi: 10.1016/j.bbamem.2020.183404. Epub 2020 Jun 23.
3
Integrated solid-state NMR and molecular dynamics modeling determines membrane insertion of human β-defensin analog.整合固态 NMR 和分子动力学建模确定人 β-防御素类似物的膜插入。
Commun Biol. 2019 Nov 1;2:402. doi: 10.1038/s42003-019-0653-6. eCollection 2019.
4
Prebiotic amino acids bind to and stabilize prebiotic fatty acid membranes.益生元氨基酸与益生元脂肪酸膜结合并稳定其结构。
Proc Natl Acad Sci U S A. 2019 Aug 27;116(35):17239-17244. doi: 10.1073/pnas.1900275116. Epub 2019 Aug 12.
5
Residue-specific structures and membrane locations of pH-low insertion peptide by solid-state nuclear magnetic resonance.通过固态核磁共振确定pH低插入肽的残基特异性结构和膜定位
Nat Commun. 2015 Jul 21;6:7787. doi: 10.1038/ncomms8787.
6
Magic angle spinning NMR of viruses.病毒的魔角旋转核磁共振
Prog Nucl Magn Reson Spectrosc. 2015 Apr;86-87:21-40. doi: 10.1016/j.pnmrs.2015.02.003. Epub 2015 Feb 16.
7
REDOR solid-state NMR as a probe of the membrane locations of membrane-associated peptides and proteins.旋转回波双共振固态核磁共振技术作为一种探测膜相关肽和蛋白质在膜中位置的手段。
J Magn Reson. 2015 Apr;253:154-65. doi: 10.1016/j.jmr.2014.12.020.
8
Multiple locations of peptides in the hydrocarbon core of gel-phase membranes revealed by peptide (13)C to lipid (2)H rotational-echo double-resonance solid-state nuclear magnetic resonance.通过肽（¹³C）到脂质（²H）旋转回波双共振固态核磁共振揭示凝胶相膜烃核中肽的多个位置。
Biochemistry. 2015 Jan 27;54(3):677-84. doi: 10.1021/bi501211x. Epub 2015 Jan 9.
9
Solid-state nuclear magnetic resonance measurements of HIV fusion peptide 13CO to lipid 31P proximities support similar partially inserted membrane locations of the α helical and β sheet peptide structures.固态核磁共振测量 HIV 融合肽 13CO 与脂质 31P 的接近程度，支持 α 螺旋和 β 片肽结构的类似部分插入膜位置。
J Phys Chem A. 2013 Oct 3;117(39):9848-59. doi: 10.1021/jp312845w. Epub 2013 Feb 28.

本文引用的文献

1
Structure of the amantadine binding site of influenza M2 proton channels in lipid bilayers.流感 M2 质子通道在脂质双层中的金刚烷胺结合位点的结构。
Nature. 2010 Feb 4;463(7281):689-92. doi: 10.1038/nature08722.
2
Synthetic fusion peptides of tick-borne encephalitis virus as models for membrane fusion.蜱传脑炎病毒的合成融合肽作为膜融合模型。
Biochemistry. 2010 Jan 19;49(2):287-96. doi: 10.1021/bi9017895.
3
A strong correlation between fusogenicity and membrane insertion depth of the HIV fusion peptide.HIV融合肽的融合活性与膜插入深度之间存在强相关性。
Proc Natl Acad Sci U S A. 2009 Sep 8;106(36):15314-9. doi: 10.1073/pnas.0907360106. Epub 2009 Aug 24.
4
Structure and dynamics of membrane proteins by magic angle spinning solid-state NMR.通过魔角旋转固态核磁共振研究膜蛋白的结构与动力学
Annu Rev Biophys. 2009;38:385-403. doi: 10.1146/annurev.biophys.050708.133719.
5
Structures and mechanisms of viral membrane fusion proteins: multiple variations on a common theme.病毒膜融合蛋白的结构与机制：同一主题的多种变体
Crit Rev Biochem Mol Biol. 2008 May-Jun;43(3):189-219. doi: 10.1080/10409230802058320.
6
Solid-state NMR spectroscopy of human immunodeficiency virus fusion peptides associated with host-cell-like membranes: 2D correlation spectra and distance measurements support a fully extended conformation and models for specific antiparallel strand registries.与宿主细胞样膜相关的人类免疫缺陷病毒融合肽的固态核磁共振光谱：二维相关光谱和距离测量支持完全伸展的构象以及特定反平行链排列的模型。
J Am Chem Soc. 2008 Apr 23;130(16):5459-71. doi: 10.1021/ja077302m. Epub 2008 Mar 28.
7
Membrane-bound conformation and topology of the antimicrobial peptide tachyplesin I by solid-state NMR.利用固态核磁共振技术研究抗菌肽鲎素I的膜结合构象和拓扑结构
Biochemistry. 2006 Nov 7;45(44):13323-30. doi: 10.1021/bi061424u.
8
Conformational flexibility and strand arrangements of the membrane-associated HIV fusion peptide trimer probed by solid-state NMR spectroscopy.通过固态核磁共振光谱法探测膜相关HIV融合肽三聚体的构象灵活性和链排列
Biochemistry. 2006 Oct 31;45(43):12960-75. doi: 10.1021/bi0615902.
9
The HIV lipidome: a raft with an unusual composition.HIV脂质组：一个成分独特的脂筏。
Proc Natl Acad Sci U S A. 2006 Feb 21;103(8):2641-6. doi: 10.1073/pnas.0511136103. Epub 2006 Feb 15.
10
Secondary structure and lipid contact of a peptide antibiotic in phospholipid bilayers by REDOR.利用旋转回波双共振技术研究肽抗生素在磷脂双分子层中的二级结构和脂质相互作用
Biophys J. 2004 Jul;87(1):662-74. doi: 10.1529/biophysj.103.032706.