病毒的魔角旋转核磁共振
Magic angle spinning NMR of viruses.
作者信息
Quinn Caitlin M, Lu Manman, Suiter Christopher L, Hou Guangjin, Zhang Huilan, Polenova Tatyana
机构信息
Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States; Pittsburgh Center for HIV Protein Interactions, University of Pittsburgh School of Medicine, 1051 Biomedical Science Tower 3, 3501 Fifth Ave., Pittsburgh, PA 15261, United States.
Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716, United States.
出版信息
Prog Nucl Magn Reson Spectrosc. 2015 Apr;86-87:21-40. doi: 10.1016/j.pnmrs.2015.02.003. Epub 2015 Feb 16.
Abstract
Viruses, relatively simple pathogens, are able to replicate in many living organisms and to adapt to various environments. Conventional atomic-resolution structural biology techniques, X-ray crystallography and solution NMR spectroscopy provided abundant information on the structures of individual proteins and nucleic acids comprising viruses; however, viral assemblies are not amenable to analysis by these techniques because of their large size, insolubility, and inherent lack of long-range order. In this article, we review the recent advances in magic angle spinning NMR spectroscopy that enabled atomic-resolution analysis of structure and dynamics of large viral systems and give examples of several exciting case studies.
摘要
病毒是相对简单的病原体，能够在许多生物体中复制并适应各种环境。传统的原子分辨率结构生物学技术，即X射线晶体学和溶液核磁共振光谱学，提供了关于构成病毒的单个蛋白质和核酸结构的丰富信息；然而，病毒组装体由于其尺寸大、不溶性以及固有的缺乏长程有序性，不适用于这些技术的分析。在本文中，我们回顾了魔角旋转核磁共振光谱学的最新进展，这些进展使得对大型病毒系统的结构和动力学进行原子分辨率分析成为可能，并给出了几个令人兴奋的案例研究示例。
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