Rivera César, Monsalve Francisco, Suazo Iván, Becerra Javiera
Unit of Histology and Embryology, Department of Basic Biomedical Sciences, Faculty of Health Sciences; ; Master Program in Biomedical Sciences, Oral Pathology mention, University of Talca, Talca;
Exp Ther Med. 2012 Nov;4(5):883-888. doi: 10.3892/etm.2012.675. Epub 2012 Aug 20.
This study aimed to examine the effect of chronic restraint stress (RS) on the severity of experimental periodontal disease in rats. A total of 32 male Sprague Dawley (SD) rats were divided into four groups: i) Rats receiving two treatment regimens, chronic stress induced by movement restriction in acrylic cylinders for 1-1.5 h daily and induction of experimental periodontal disease, using a nylon ligature which was placed around the first left mandibular molars (n=8); ii) induction of periodontal disease, without RS (n=8); iii) RS (n=8) and iv) control (n=8). After 15 days, blood samples were obtained, and blood glucose levels and the corticosterone concentration were measured as stress markers. The severity of periodontal disease was analyzed according to the level of gingival and bone inflammation, leading to compromise of the teeth involved. Chronic stress was induced with movement restriction (P≤0.05, Mann-Whitney U-test) and increased the severity (P≤0.05, Mann-Whitney U-test) of experimental perio dontal disease in rats, according to the level of gingival and bone inflammation around the first left mandibular molars. The results of the present study showed that RS modulates periodontal inflammation and that the rat model described herein is suitable for investigating the association between stress and periodontal disease.
本研究旨在探讨慢性束缚应激（RS）对大鼠实验性牙周病严重程度的影响。总共32只雄性斯普拉格-道利（SD）大鼠被分为四组：i）接受两种处理方案的大鼠，即每天在丙烯酸圆柱体内通过运动限制诱导1 - 1.5小时的慢性应激，并使用尼龙结扎线环绕左侧第一下颌磨牙诱导实验性牙周病（n = 8）；ii）仅诱导牙周病，无RS（n = 8）；iii）RS组（n = 8）；iv）对照组（n = 8）。15天后，采集血样，测量血糖水平和皮质酮浓度作为应激指标。根据牙龈和骨炎症水平分析牙周病的严重程度，这会导致受累牙齿受到损害。通过运动限制诱导慢性应激（P≤0.05，曼-惠特尼U检验），并且根据左侧第一下颌磨牙周围牙龈和骨炎症水平，增加了大鼠实验性牙周病的严重程度（P≤0.05，曼-惠特尼U检验）。本研究结果表明，RS可调节牙周炎症，并且本文所述的大鼠模型适用于研究应激与牙周病之间的关联。
