J Dent Res. 2011 Jun;90(6):799-803. doi: 10.1177/0022034511399911. Epub 2011 Mar 10.
Pathogenic processes have been identified that could associate chronic stress and cancer, but these findings have not been observed in oral cancer. This study examined the role of chronic restraint stress on the incidence and severity of OSCC induced with 4-nitroquinoline-1-oxide (4-NQO) in the tongues of CF-1 mice. One hundred twenty CF-1 male mice were divided into 4 groups: (A) received two treatments - restraint stress and induction of chemical carcinogenesis (n = 50); (B) induction of chemical carcinogenesis, without restraint stress (n = 50); (C) restraint stress (n = 10); and (D) control (n = 10). After 30 weeks, tongues were dissected and analyzed by conventional histopathology. The severity of OSSC was analyzed according to the International Histological Classification of Tumors and Bryne's Multifactorial Grading System for the Invasive Tumor Front (ITF). Chronic stress induction was confirmed by plasma corticosterone levels. Results showed that chronic stress was induced with movement restriction (p ≤ 0.05, Mann-Whitney U-test). However, chronic stress did not increase the incidence (p > 0.05, Chi-square) or severity (p > 0.05, Mann-Whitney U-test) of the 4-NQO-induced OSSC in the tongues of CF-1 mice. These results suggest that there is no relationship between chronic stress (induced in mice by restraint) and the incidence and severity of OSSC.
已经确定了与慢性应激和癌症相关的发病机制，但这些发现尚未在口腔癌中观察到。本研究探讨了慢性束缚应激在 4-硝基喹啉-1-氧化物（4-NQO）诱导 CF-1 小鼠舌部口腔鳞状细胞癌（OSCC）发生和严重程度中的作用。将 120 只 CF-1 雄性小鼠分为 4 组：（A）接受两种处理 - 束缚应激和化学致癌诱导（n = 50）；（B）化学致癌诱导，无束缚应激（n = 50）；（C）束缚应激（n = 10）；和（D）对照组（n = 10）。30 周后，将舌头解剖并进行常规组织病理学分析。根据国际肿瘤组织学分类和 Bryne 浸润性肿瘤前缘多因素分级系统（ITF）分析 OSSC 的严重程度。通过血浆皮质酮水平确认慢性应激诱导。结果表明，通过运动限制诱导了慢性应激（p ≤ 0.05，Mann-Whitney U 检验）。然而，慢性应激并没有增加 CF-1 小鼠舌部 4-NQO 诱导的 OSSC 的发生率（p > 0.05，卡方检验）或严重程度（p > 0.05，Mann-Whitney U 检验）。这些结果表明，慢性应激（通过束缚在小鼠中诱导）与 OSSC 的发生率和严重程度之间没有关系。
