Instituto de Ensino e Pesquisa da Santa Casa de Belo Horizonte, Minas Gerais, Brazil.
Hum Immunol. 2013 Apr;74(4):452-8. doi: 10.1016/j.humimm.2012.11.025. Epub 2012 Dec 8.
Besides HPV infection, the progression to cervical cancer also depends on the host immune response. HLA-G molecules are involved in the inhibition of cell-mediated immune responses and may permit the development of an infection in the female cervical tract. The aim of this study was to explore the possible influence of the two HLA-G polymorphisms located on the 3'UTR on the susceptibility to cervical cancer and risk factors in Brazilian patients. Polymorphism analysis (14 bp In/Del and +3142C/G) was performed by PCR. A total of 105 cervical samples were tested, 50 without lesions and 55 with lesions; 22 with high grade (HSIL) and 33 with invasive cancer (ICC). The polymorphisms (∗)Del/Del was associated with a decreased risk of developing ICC in smokers and (∗)In and (∗)In/In were associated with an increased risk of HSIL and a higher risk of ICC in smokers. The genotype (∗)In/Del was associated with the increased risk of HSIL only among women with a family history of cancer. The haplotypes (∗)In/G and (∗)Del/G were associated with increased and decreased risk of HSIL and cervical cancer, respectively. In conclusion, the 3'UTR of HLA-G is associated with an increased risk of developing cervical cancer, especially in smokers.
除 HPV 感染外，宫颈癌的进展还取决于宿主的免疫反应。HLA-G 分子参与抑制细胞介导的免疫反应，可能允许女性宫颈部位的感染发展。本研究旨在探讨位于 3'UTR 上的两个 HLA-G 多态性对巴西患者宫颈癌易感性和危险因素的可能影响。通过 PCR 进行多态性分析（14 bp In/Del 和 +3142C/G）。共检测了 105 个宫颈样本，50 个无病变，55 个有病变；22 个为高级别病变（HSIL），33 个为浸润性癌（ICC）。（∗）Del/Del 多态性与吸烟者 ICC 发病风险降低相关，（∗）In 和（∗）In/In 多态性与 HSIL 发病风险增加和吸烟者 ICC 发病风险增加相关。（∗）In/Del 基因型仅与有癌症家族史的女性发生 HSIL 的风险增加相关。（∗）In/G 和（∗）Del/G 单倍型分别与 HSIL 和宫颈癌发病风险增加和降低相关。总之，HLA-G 的 3'UTR 与宫颈癌发病风险增加相关，尤其是在吸烟者中。
