Department of Paediatric Dentistry, School of Dentistry, University of Granada, Campus de Cartuja, Colegio Máximo s/n, Granada, Spain.
Med Oral Patol Oral Cir Bucal. 2013 Mar 1;18(2):e319-24. doi: 10.4317/medoral.18425.
The aim of this study was to evaluate, ex vivo, bacterial coronal leakage with different antimicrobial agents applied to the dentine for indirect pulp treatment (IPT).
Sixty extracted teeth were prepared and randomly distributed into 5 groups (n=10): Group 1: no antimicrobial dentine treatment; group 2: 1% chlorhexidine (CHX)+1% thymol varnish (Cervitec); group 3: 2 % CHX solution; group 4: 40% CHX varnish (EC40) and group 5: Clearfil Protect Bond (CPB). Ten teeth served as controls. The teeth were restored using a resin-modified glass ionomer cement (GIC) and then mounted in a two-chamber device. The coronal access was exposed to Streptococcus mutans for 45 days. The appearance of turbidity in the BHI broth of the lower chamber was considered as specimen leakage.
Survival analysis, determined by non parametric Kaplan-Meier and log-rank tests, showed that the best results were for groups EC40+GIC and GIC alone; yet there were not statistically significant differences between them. All specimens of CPB+GIC and 2% CHX+GIC, leaked at 45 days.
In IPT the use of GIC without pretreatment of the dentine and pretreatment with 40% CHX varnish resulted in a significant delay of bacterial coronal leakage.
本研究旨在评估不同抗菌剂应用于间接牙髓治疗（IPT）牙本质时的冠向细菌渗漏情况，采用离体实验方法。
60 颗离体牙被制备并随机分为 5 组（n=10）：第 1 组：不进行抗菌牙本质处理；第 2 组：1%洗必泰（CHX）+1%麝香草酚漆（Cervitec）；第 3 组：2%CHX 溶液；第 4 组：40%CHX 漆（EC40）；第 5 组：Clearfil Protect Bond（CPB）。另有 10 颗牙作为对照组。牙齿用树脂改良型玻璃离子水门汀（GIC）修复，然后安装在双室装置中。冠部开口暴露于变异链球菌 45 天。下室 BHI 肉汤出现浑浊被视为样本渗漏。
生存分析，采用非参数 Kaplan-Meier 和对数秩检验确定，结果表明 EC40+GIC 和 GIC 组的结果最好；但它们之间没有统计学上的显著差异。所有 CPB+GIC 和 2%CHX+GIC 标本在 45 天时均发生渗漏。
在 IPT 中，不预处理牙本质并用 40%CHX 漆预处理，使用 GIC 可显著延迟细菌冠向渗漏。
