Department of Pediatric Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand.
Oral Biology Research Center, Faculty of Dentistry, Chulalongkorn University, 34 Pathumwan, Bangkok, 10330, Thailand.
BMC Oral Health. 2022 Mar 5;22(1):60. doi: 10.1186/s12903-022-02098-1.
To compare the mean mineral density (MMD) and examine the remineralization of carious dentin after cavity disinfection with chlorhexidine gluconate (CHX) and restoration with high viscosity glass ionomer cement (H-GIC) in vitro.
Selective caries removal to leathery dentin was performed in 40 extracted primary molars. The samples were scanned using micro-computed tomography (micro-CT) to determine the MMD baseline and randomly divided into 4 groups (n = 10): Equia™ group, applied dentin conditioner and restored with H-GIC (Equia Forte™), CHX-Equia™ group, disinfected the cavity with 2% CHX before applying dentin conditioner and restored with H-GIC (Equia Forte™), Ketac™ group, restored with H-GIC (Ketac Universal™) and CHX-Ketac™ group, disinfected the cavity with 2% CHX before restored with H-GIC (Ketac Universal™). The samples underwent micro-CT scanning post-restoration and post-pH-cycling to determine their respective MMDs. One sample from each group was randomly selected to analyze by scanning electron microscopy (SEM).
The MMD gain in the 4 groups post-restoration was significantly different between the Equia™ and CHX-Ketac™ groups (oneway ANOVA with Post hoc (Tukey) test, P = 0.045). There was a significant difference in MMD gain post-restoration between the Equia™ and CHX-Equia™ groups (Independent t-test, P = 0.046). However, the Ketac™ and CHX-Ketac™ group's MMD were similar. The SEM images revealed that the CHX-Ketac™ group had the smallest dentinal tubule orifices and the thickest intertubular dentin among the groups. However, the CHX-Equia™ group had thicker intertubular dentin than the Equia™ group.
Applying 2% CHX on demineralized dentin enhances the remineralization of the dentin beneath the restoration.
本研究旨在通过体外实验比较盐酸葡萄糖酸氯己定（CHX）消毒窝洞和高粘度玻璃离子水门汀（H-GIC）修复后牙本质的平均矿化密度（MMD），并观察牙本质再矿化情况。
从 40 颗拔除的乳磨牙中选择性地去除到皮革状牙本质。使用微计算机断层扫描（micro-CT）对样本进行扫描，以确定 MMD 基线，并将样本随机分为 4 组（n=10）：Equia™组，应用牙本质调节剂并用 H-GIC（Equia Forte™）修复；CHX-Equia™组，先用 2% CHX 消毒窝洞，再应用牙本质调节剂并用 H-GIC（Equia Forte™）修复；Ketac™组，用 H-GIC（Ketac Universal™）修复；CHX-Ketac™组，先用 2% CHX 消毒窝洞，再用 H-GIC（Ketac Universal™）修复。修复后和 pH 循环后，对样本进行 micro-CT 扫描，以确定各自的 MMD。从每组中随机选择一个样本进行扫描电子显微镜（SEM）分析。
4 组修复后 MMD 的增加有显著差异（单因素方差分析，Post hoc（Tukey）检验，P=0.045）。Equia™组和 CHX-Ketac™组间修复后 MMD 的增加有显著差异（独立样本 t 检验，P=0.046）。而 Ketac™组和 CHX-Ketac™组间 MMD 相似。SEM 图像显示，CHX-Ketac™组牙本质小管口最小，各组中相邻小管间牙本质最厚。然而，CHX-Equia™组的相邻小管间牙本质比 Equia™组厚。
将 2% CHX 应用于脱矿牙本质可增强修复下牙本质的再矿化。
