Hartmann-Fritsch Fabienne, Biedermann Thomas, Braziulis Erik, Luginbühl Joachim, Pontiggia Luca, Böttcher-Haberzeth Sophie, van Kuppevelt Toin H, Faraj Kaeuis A, Schiestl Clemens, Meuli Martin, Reichmann Ernst
Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, Switzerland.
Department of Surgery, Paediatric Burn Centre, Plastic and Reconstructive Surgery, University Children's Hospital Zurich, Switzerland.
J Tissue Eng Regen Med. 2016 Jan;10(1):81-91. doi: 10.1002/term.1665. Epub 2012 Dec 10.
Extensive full-thickness skin loss, associated with deep burns or other traumata, represents a major clinical problem that is far from being solved. A promising approach to treat large skin defects is the use of tissue-engineered full-thickness skin analogues with nearly normal anatomy and function. In addition to excellent biological properties, such skin substitutes should exhibit optimal structural and mechanical features. This study aimed to test novel dermo-epidermal skin substitutes based on collagen type I hydrogels, physically strengthened by two types of polymeric net-like meshes. One mesh has already been used in clinical trials for treating inguinal hernia; the second one is new but consists of a FDA-approved polymer. Both meshes were integrated into collagen type I hydrogels and dermo-epidermal skin substitutes were generated. Skin substitutes were transplanted onto immuno-incompetent rats and analyzed after distinct time periods. The skin substitutes homogeneously developed into a well-stratified epidermis over the entire surface of the grafts. The epidermis deposited a continuous basement membrane and dermo-epidermal junction, displayed a well-defined basal cell layer, about 10 suprabasal strata and a stratum corneum. Additionally, the dermal component of the grafts was well vascularized.
大面积全层皮肤缺失，与深度烧伤或其他创伤相关，是一个尚未得到解决的重大临床问题。一种治疗大面积皮肤缺损的有前景的方法是使用具有近乎正常解剖结构和功能的组织工程全层皮肤类似物。除了优异的生物学特性外，这种皮肤替代物还应展现出最佳的结构和力学特征。本研究旨在测试基于I型胶原水凝胶的新型真皮 - 表皮皮肤替代物，通过两种类型的聚合物网状网进行物理强化。一种网已用于治疗腹股沟疝的临床试验；另一种是新型的，但由一种FDA批准的聚合物组成。两种网都整合到I型胶原水凝胶中，并生成真皮 - 表皮皮肤替代物。将皮肤替代物移植到免疫缺陷大鼠身上，并在不同时间段后进行分析。皮肤替代物在移植物的整个表面均匀地发育成分层良好的表皮。表皮沉积了连续的基底膜和真皮 - 表皮连接，显示出明确的基底细胞层、约10层基底上层和角质层。此外，移植物的真皮成分血管化良好。
