缺铁性贫血患者脱落颊黏膜细胞的细胞形态计量分析研究
A study on cytomorphometric analysis of exfoliative buccal cells in iron deficiency anemic patients.
作者信息
Sumanthi J, Reddy G Sridhar, Anuradha C H, Sekhar P Chandhra, Prasad L Krishna, Reddy B V Ramana
机构信息
Department of Oral Pathology, Lenora Institute of Dental Sciences, Rajahmundry, Andhra Pradesh, India.
出版信息
Contemp Clin Dent. 2012 Sep;3(Suppl 2):S156-9. doi: 10.4103/0976-237X.101071.
AIM
The aim was to evaluate the quantitative changes in nuclear diameter (ND), cytoplasmic diameter (CD) and nuclear/cytoplasmic ratio (N/C) in cytological buccal smears of iron deficiency anemic patients by comparing with normal healthy individuals.
MATERIALS AND METHODS
The study group consisted of 40 healthy individuals and 40 iron deficiency anemic patients who were selected on clinical history, hematological investigations, and confirmed by serum ferritin levels. Exfoliative buccal smears stained with PAP stain were evaluated for cytoplasimic, nuclear diameters, and nuclear/cytoplasmic ratios (N/C) using Image Proexpress Version 6.0 image analysis system. All the parameters were statistically analyzed by using unpaired 't' test.
RESULTS
A significant increase is seen in the average nuclear diameter (ND) and N/C ratio of the anemic group when compared to the control group. The average cytoplasmic diameter (CD) did not show any statistical difference among the two groups.
CONCLUSION
Oral exfoliative cytological techniques could possibly be a noninvasive alternative diagnostic tool for iron deficiency anemia.
目的
通过与正常健康个体进行比较，评估缺铁性贫血患者口腔细胞学涂片的细胞核直径（ND）、细胞质直径（CD）和核质比（N/C）的定量变化。
材料与方法
研究组包括40名健康个体和40名缺铁性贫血患者，这些患者通过临床病史、血液学检查进行筛选，并经血清铁蛋白水平确诊。使用Image Proexpress 6.0版图像分析系统对经巴氏染色的口腔脱落涂片进行细胞质、细胞核直径及核质比（N/C）评估。所有参数均采用非配对“t”检验进行统计学分析。
结果
与对照组相比，贫血组的平均细胞核直径（ND）和N/C比显著增加。两组间平均细胞质直径（CD）未显示出任何统计学差异。
结论
口腔脱落细胞学技术可能成为缺铁性贫血的一种非侵入性替代诊断工具。
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