Ohta M, Ohta K, Itoh N, Kurobe M, Hayashi K, Nishitani H
Department of Clinical Research Center, Utano National Hospital, Kyoto, Japan.
Clin Chim Acta. 1990 Mar 15;187(3):255-64. doi: 10.1016/0009-8981(90)90110-e.
We recently reported a strong association between the occurrence of anti-skeletal muscle (SM) antibodies and the presence of thymoma in patients with myasthenia gravis (MG). To further examine the immunoreactivity of MG sera against human muscle antigens, we developed a solid-phase radioimmunoassay (RIA) using purified muscle antigens and a Western blotting analysis in MG sera with high titers of anti-SM antibodies. Our results showed that MG patients with thymoma (thymoma group) had markedly high titers of anti-myosin and anti-actomyosin antibodies than those without thymoma (non-thymoma group). Furthermore, a close correlation was found between titers of anti-SM, anti-myosin and anti-actomyosin antibodies. The antibody titers against actin, alpha-actinin and tropomyosin were all low and did not correlate with titers of anti-SM antibodies. But, significant levels of these three antibodies were found in the thymoma group. By a Western blotting analysis, immunoreactivity of sera from the thymoma group appeared to be predominantly directed against myosin, actin and alpha-actinin.
我们最近报道了重症肌无力（MG）患者中抗骨骼肌（SM）抗体的出现与胸腺瘤的存在之间存在密切关联。为了进一步研究MG血清对人肌肉抗原的免疫反应性，我们使用纯化的肌肉抗原开发了一种固相放射免疫测定（RIA），并对具有高滴度抗SM抗体的MG血清进行了蛋白质印迹分析。我们的结果表明，患有胸腺瘤的MG患者（胸腺瘤组）的抗肌球蛋白和抗肌动球蛋白抗体滴度明显高于无胸腺瘤的患者（非胸腺瘤组）。此外，发现抗SM、抗肌球蛋白和抗肌动球蛋白抗体的滴度之间存在密切相关性。针对肌动蛋白、α-辅肌动蛋白和原肌球蛋白的抗体滴度均较低，且与抗SM抗体滴度无关。但是，在胸腺瘤组中发现了这三种抗体的显著水平。通过蛋白质印迹分析，胸腺瘤组血清的免疫反应性似乎主要针对肌球蛋白、肌动蛋白和α-辅肌动蛋白。
