DNA 适体介导的细胞靶向。
DNA aptamer-mediated cell targeting.
机构信息
Department of Chemistry, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200, USA.
出版信息
Angew Chem Int Ed Engl. 2013 Jan 28;52(5):1472-6. doi: 10.1002/anie.201207063. Epub 2012 Dec 11.
Abstract
One important issue using cells as therapeutics is targeted delivery. Engineering cell surfaces to improve delivery efficiency is thus of great interest. Here we report a simple, efficient and effective way to modify the cell surface with target-specific ligands, i.e., DNA aptamers, while minimizing the effects on the modified cells. We demonstrated that after incubating with lipo-aptamer probes (shown in expansion), immune cells (red) recognize cancer cells (blue) in the cell mixture, and kill cancer cells.
摘要
使用细胞作为治疗方法的一个重要问题是靶向递送。因此，工程细胞表面以提高递送效率具有重要意义。在这里，我们报告了一种简单、高效和有效的方法，即用靶向配体（即 DNA 适体）来修饰细胞表面，同时最大限度地减少对修饰细胞的影响。我们证明，在用脂质体适体探针孵育后（如图中扩展所示），免疫细胞（红色）能够识别细胞混合物中的癌细胞（蓝色）并杀死癌细胞。
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