DNA 适体介导的细胞靶向。
DNA aptamer-mediated cell targeting.
机构信息
Department of Chemistry, Shands Cancer Center, Center for Research at the Bio/nano Interface, University of Florida, Gainesville, FL 32611-7200, USA.
出版信息
Angew Chem Int Ed Engl. 2013 Jan 28;52(5):1472-6. doi: 10.1002/anie.201207063. Epub 2012 Dec 11.
Abstract
One important issue using cells as therapeutics is targeted delivery. Engineering cell surfaces to improve delivery efficiency is thus of great interest. Here we report a simple, efficient and effective way to modify the cell surface with target-specific ligands, i.e., DNA aptamers, while minimizing the effects on the modified cells. We demonstrated that after incubating with lipo-aptamer probes (shown in expansion), immune cells (red) recognize cancer cells (blue) in the cell mixture, and kill cancer cells.
摘要
使用细胞作为治疗方法的一个重要问题是靶向递送。因此，工程细胞表面以提高递送效率具有重要意义。在这里，我们报告了一种简单、高效和有效的方法，即用靶向配体（即 DNA 适体）来修饰细胞表面，同时最大限度地减少对修饰细胞的影响。我们证明，在用脂质体适体探针孵育后（如图中扩展所示），免疫细胞（红色）能够识别细胞混合物中的癌细胞（蓝色）并杀死癌细胞。
相似文献
1
DNA aptamer-mediated cell targeting.DNA 适体介导的细胞靶向。
Angew Chem Int Ed Engl. 2013 Jan 28;52(5):1472-6. doi: 10.1002/anie.201207063. Epub 2012 Dec 11.
2
Aptamer-target binding triggered molecular mediation of singlet oxygen generation.适配体-靶点结合触发单线态氧生成的分子介导。
Chem Asian J. 2010 Apr 1;5(4):783-6. doi: 10.1002/asia.200900545.
3
Aptamer-Directed Protein-Specific Multiple Modifications of Membrane Glycoproteins on Living Cells.适体导向的活细胞表面糖蛋白的蛋白特异性多重修饰。
ACS Appl Mater Interfaces. 2020 Aug 26;12(34):37845-37850. doi: 10.1021/acsami.0c07004. Epub 2020 Aug 13.
4
Molecular engineering of DNA bases: building block for functional molecular probes in biomedicine.DNA碱基的分子工程：生物医学中功能性分子探针的构建模块。
Nucleic Acids Symp Ser (Oxf). 2008(52):65-6. doi: 10.1093/nass/nrn033.
5
Aptamer-based optical probes with separated molecular recognition and signal transduction modules.具有分离的分子识别和信号转导模块的基于适配体的光学探针。
J Am Chem Soc. 2008 Feb 27;130(8):2380-1. doi: 10.1021/ja076787b. Epub 2008 Feb 5.
6
Rapid Detection of Mycoplasma-Infected Cells by an ssDNA Aptamer Probe.通过 ssDNA 适体探针快速检测支原体感染细胞。
ACS Sens. 2019 Aug 23;4(8):2028-2038. doi: 10.1021/acssensors.9b00582. Epub 2019 Aug 12.
7
Supersandwich cytosensor for selective and ultrasensitive detection of cancer cells using aptamer-DNA concatamer-quantum dots probes.基于适体-DNA 连接子-量子点探针的三明治型细胞传感器用于选择性和超高灵敏度检测癌细胞。
Anal Chem. 2013 Mar 19;85(6):3385-92. doi: 10.1021/ac303789x. Epub 2013 Mar 4.
8
Aptamer-DNA concatamer-quantum dots based electrochemical biosensing strategy for green and ultrasensitive detection of tumor cells via mercury-free anodic stripping voltammetry.基于适配体-DNA 连接物-量子点的电化学生物传感策略，通过无汞阳极溶出伏安法实现对肿瘤细胞的绿色、超灵敏检测。
Biosens Bioelectron. 2019 Feb 1;126:261-268. doi: 10.1016/j.bios.2018.09.076. Epub 2018 Sep 21.
9
Engineering dendritic aptamer assemblies as superior inhibitors of protein function.设计树枝状适体组装体作为蛋白质功能的高效抑制剂。
Chem Asian J. 2010 Jan 4;5(1):56-9. doi: 10.1002/asia.200900421.
10
High affinity truncated DNA aptamers for the development of fluorescence based progesterone biosensors.用于开发基于荧光的孕酮生物传感器的高亲和力截短型DNA适配体。
Anal Biochem. 2017 May 15;525:78-84. doi: 10.1016/j.ab.2017.02.014. Epub 2017 Feb 24.
引用本文的文献
1
Engineered extracellular vesicles with sequential cell recruitment and osteogenic functions to effectively promote senescent bone repair.具有顺序性细胞募集和成骨功能的工程化细胞外囊泡可有效促进衰老骨骼修复。
J Nanobiotechnology. 2025 Feb 12;23(1):107. doi: 10.1186/s12951-025-03168-6.
2
Natural biomolecules for cell-interface engineering.用于细胞界面工程的天然生物分子。
Chem Sci. 2025 Jan 28;16(7):3019-3044. doi: 10.1039/d4sc08422e. eCollection 2025 Feb 12.
3
Controlling Cell Interactions with DNA Directed Assembly.通过DNA定向组装控制细胞相互作用。
Adv Healthc Mater. 2024 Dec;13(32):e2402876. doi: 10.1002/adhm.202402876. Epub 2024 Oct 14.
4
Developing an erythrocyte‒MHC-I conjugate for cancer treatment.开发用于癌症治疗的红细胞-MHC-I缀合物。
Cell Discov. 2024 Oct 1;10(1):99. doi: 10.1038/s41421-024-00713-9.
5
Designer cellular spheroids with DNA origami for drug screening.利用 DNA 折纸术设计细胞球体用于药物筛选。
Sci Adv. 2024 Jul 19;10(29):eado9880. doi: 10.1126/sciadv.ado9880.
6
DNA self-assembly nanoflower reverse P-glycoprotein mediated drug resistance in chronic myelogenous leukemia therapy.DNA自组装纳米花逆转慢性粒细胞白血病治疗中P-糖蛋白介导的耐药性。
Front Bioeng Biotechnol. 2023 Aug 21;11:1265199. doi: 10.3389/fbioe.2023.1265199. eCollection 2023.
7
Nucleic acid amphiphiles: Synthesis, properties, and applications.核酸两亲分子：合成、性质及应用
Mol Ther Nucleic Acids. 2023 Jun 3;33:144-163. doi: 10.1016/j.omtn.2023.05.022. eCollection 2023 Sep 12.
8
Lipid-linked nucleoside triphosphates for enzymatic synthesis of hydrophobic oligonucleotides with enhanced membrane anchoring efficiency.用于酶促合成具有增强膜锚定效率的疏水寡核苷酸的脂质连接核苷三磷酸。
Chem Sci. 2023 Mar 20;14(15):4059-4069. doi: 10.1039/d2sc06718h. eCollection 2023 Apr 12.
9
Could artificial intelligence revolutionize the development of nanovectors for gene therapy and mRNA vaccines?人工智能能否彻底改变用于基因治疗和mRNA疫苗的纳米载体的研发？
Nano Today. 2022 Dec;47. doi: 10.1016/j.nantod.2022.101665. Epub 2022 Nov 7.
10
Spinal cord tissue engineering via covalent interaction between biomaterials and cells.通过生物材料与细胞的共价相互作用进行脊髓组织工程。
Sci Adv. 2023 Feb 10;9(6):eade8829. doi: 10.1126/sciadv.ade8829. Epub 2023 Feb 8.
本文引用的文献
1
Engineered cell homing.工程化细胞归巢。
Blood. 2011 Dec 15;118(25):e184-91. doi: 10.1182/blood-2010-10-311464. Epub 2011 Oct 27.
2
Chimeric antigen receptor-modified T cells in chronic lymphoid leukemia.嵌合抗原受体修饰的 T 细胞治疗慢性淋巴细胞白血病。
N Engl J Med. 2011 Aug 25;365(8):725-33. doi: 10.1056/NEJMoa1103849. Epub 2011 Aug 10.
3
Aptamers as therapeutics.适配子疗法。
Nat Rev Drug Discov. 2010 Jul;9(7):537-50. doi: 10.1038/nrd3141.
4
Redirecting T-cell specificity by introducing a tumor-specific chimeric antigen receptor.通过引入肿瘤特异性嵌合抗原受体来改变 T 细胞的特异性。
Blood. 2010 Aug 19;116(7):1035-44. doi: 10.1182/blood-2010-01-043737. Epub 2010 May 3.
5
DNA-based micelles: synthesis, micellar properties and size-dependent cell permeability.基于 DNA 的胶束：合成、胶束性质和尺寸依赖性细胞通透性。
Chemistry. 2010 Mar 22;16(12):3791-7. doi: 10.1002/chem.200901546.
6
Molecular recognition of acute myeloid leukemia using aptamers.使用适配体对急性髓系白血病进行分子识别。
Leukemia. 2009 Feb;23(2):235-44. doi: 10.1038/leu.2008.335. Epub 2009 Jan 8.
7
Cell-specific aptamer probes for membrane protein elucidation in cancer cells.用于阐明癌细胞膜蛋白的细胞特异性适配体探针。
J Proteome Res. 2008 May;7(5):2133-9. doi: 10.1021/pr700894d. Epub 2008 Mar 26.
8
Cancer cell targeting using multiple aptamers conjugated on nanorods.使用缀合在纳米棒上的多种适配体进行癌细胞靶向
Anal Chem. 2008 Feb 1;80(3):567-72. doi: 10.1021/ac702322j. Epub 2008 Jan 1.
9
Selection of fluorescent aptamer beacons that light up in the presence of zinc.在锌存在时发光的荧光适体信标的筛选。
Anal Bioanal Chem. 2008 Feb;390(4):1067-75. doi: 10.1007/s00216-007-1735-8. Epub 2007 Nov 30.
10
Genetically engineered stem cells for therapeutic gene delivery.用于治疗性基因递送的基因工程干细胞。
Curr Gene Ther. 2007 Aug;7(4):249-60. doi: 10.2174/156652307781369119.
Zotero 插件