CSIR-Institute of Microbial Technology, Sector 39A, Chandigarh, India.
Biol Direct. 2012 Dec 12;7:45; discussion 45. doi: 10.1186/1745-6150-7-45.
Ethanolamine is used as an energy source by phylogenetically diverse bacteria including pathogens, by the concerted action of proteins from the eut-operon. Previous studies have revealed the presence of eutBC genes encoding ethanolamine-ammonia lyase, a key enzyme that breaks ethanolamine into acetaldehyde and ammonia, in about 100 bacterial genomes including members of gamma-proteobacteria. However, ethanolamine utilization has not been reported for any member of the Vibrio genus. Our comparative genomics study reveals the presence of genes that are involved in ethanolamine utilization in several Vibrio species. Using Vibrio alginolyticus as a model system we demonstrate that ethanolamine is better utilized as a nitrogen source than as a carbon source.
This article was reviewed by Dr. Lakshminarayan Iyer and Dr. Vivek Anantharaman (nominated by Dr. L Aravind).
乙醇胺被包括病原体在内的系统发育多样的细菌用作能量来源，这是由 eut 操纵子的蛋白质协同作用的结果。先前的研究表明，在大约 100 个细菌基因组中存在编码乙醇胺氨裂解酶的 eutBC 基因，该酶是将乙醇胺分解成乙醛和氨的关键酶，这些细菌包括γ变形菌的成员。然而，尚未报道任何弧菌属成员能够利用乙醇胺。我们的比较基因组学研究表明，在几种弧菌物种中存在参与乙醇胺利用的基因。我们使用溶藻弧菌作为模型系统证明，与作为碳源相比，乙醇胺作为氮源被更好地利用。
本文由 Lakshminarayan Iyer 博士和 Vivek Anantharaman 博士（由 L Aravind 博士提名）进行了评审。
