Zhao Yan-Song, Zhao Kan-Xing, Wang Xiao-Li, Chen Yu-Xi, Wang Li, Mu Qing-Jie
Tianjin Eye Hospital, Tianjin Medical University, Tianjin, China.
Zhongguo Dang Dai Er Ke Za Zhi. 2012 Dec;14(12):971-5.
To explore the effects of marrow mesenchymal stem cell (BMSC) transplantation on retinal cells apoptosis and changes to neurotrophin-3 (NT-3 and ciliary neurotrophic factor (CNTF) in rats with retinopathy of prematurity (ROP).
Seven-day-old Sprague-Dawley rats were randomly divided into normal control (CON), ROP, BMSC transplantation (BMSCs were transplanted 5 days after oxygen conditioning) and phosphate buffered saline (PBS) groups. The ROP model was prepared according to the classic hyperoxygen method. Seven days after transplantation, TUNEL/DAPI, NT-3/API and CNTF/DAPI double-labeled immunofluorescence were used to examine the effects of BMSC transplantation on both the apoptosis of retinal cells and the expression of NT-3 and CNTF protein in the retinal cells of the ROP rats.
Seven days after BMSC transplantation, there were few TUNEL+ DAPI+ cells observed in the CON group. There were fewer TUNEL+DAPI+ cells observed in the BMSC group than in the ROP group (P<0.01), but there was no significant difference between the ROP and PBS groups (P>0.05). There were few NT-3+DAPI+ cells and CNTF+DAPI+ cells in the CON group. There were more NT-3+DAPI+ and CNTF+DAPI+ cells in the ROP group than in the CON group, but there was no significant difference between the ROP and CON groups (P>0.05). More NT-3+DAPI+ and CNTF+DAPI+ cells were observed in the BMSC group compared with the ROP group (P<0.01), and there was no significant difference in either NT-3+DAPI+ or CNTF+DAPI+ cells between the ROP and PBS groups (P>0.05).
BMSC transplantation therapy could alleviate the apoptosis of retinal cells in ROP rats, and its mechanisms might be associated with promoting the expression of NT-3 and CNTF protein in retinal cells.
探讨骨髓间充质干细胞（BMSC）移植对早产儿视网膜病变（ROP）大鼠视网膜细胞凋亡及神经营养因子-3（NT-3）和睫状神经营养因子（CNTF）变化的影响。
将7日龄的Sprague-Dawley大鼠随机分为正常对照组（CON）、ROP组、BMSC移植组（氧诱导5天后移植BMSC）和磷酸盐缓冲液（PBS）组。按照经典的高氧法制备ROP模型。移植7天后，采用TUNEL/DAPI、NT-3/API和CNTF/DAPI双标免疫荧光法检测BMSC移植对ROP大鼠视网膜细胞凋亡及视网膜细胞中NT-3和CNTF蛋白表达的影响。
BMSC移植7天后，CON组未见TUNEL+DAPI+细胞。BMSC组TUNEL+DAPI+细胞少于ROP组（P<0.01），但ROP组与PBS组之间无显著差异（P>0.05）。CON组NT-3+DAPI+细胞和CNTF+DAPI+细胞较少。ROP组NT-3+DAPI+和CNTF+DAPI+细胞多于CON组，但ROP组与CON组之间无显著差异（P>0.05）。与ROP组相比，BMSC组观察到更多的NT-3+DAPI+和CNTF+DAPI+细胞（P<0.01），ROP组与PBS组NT-3+DAPI+或CNTF+DAPI+细胞均无显著差异（P>0.05）。
BMSC移植治疗可减轻ROP大鼠视网膜细胞凋亡，其机制可能与促进视网膜细胞中NT-3和CNTF蛋白的表达有关。
