Popescu N C, Zimonjic D, DiPaolo J A
Division of Cancer Etiology, National Cancer Institute, Bethesda, MD 20892.
Hum Genet. 1990 Apr;84(5):383-6. doi: 10.1007/BF00195804.
To evaluate the trend of viral integration in the human genome, chromosomal localization of five DNA-containing viruses compiled from literature data was compared to the location of fragile sites and proto-oncogenes. A total of 35 regionally mapped viral integration sites from tumors and transformed cells were distributed over 19 chromosomes. Of the 35 integration sites 23 (66%) were at the bands of fragile sites, and 7 were one band away (20%). This statistically defines the correlation as highly significant (P = 0.0000183, Fisher's F-test). Five integration sites did not correspond to the location of a fragile site. Thirteen integration sites and proto-oncogenes mapped at the same bands (37%), 6 (17%) were one band apart, and at 16 integration sites (46%) no proto-oncogenes were localized (P = 0.00491). Eighteen viral integration sites, fragile sites, and proto-oncogenes (51%) were localized at the same bands or one band distant. This clustering of viral integration sites, fragile sites, and proto-oncogenes is statistically highly significant (P = 0.0000118), and indicates nonrandom viral integration in the human genome.
为评估病毒整合入人类基因组的趋势，将从文献数据汇编的五种含DNA病毒的染色体定位与脆性位点和原癌基因的位置进行了比较。来自肿瘤和转化细胞的总共35个区域定位的病毒整合位点分布在19条染色体上。在这35个整合位点中，23个（66%）位于脆性位点的条带处，7个距离脆性位点条带一个条带（20%）。这在统计学上确定这种相关性非常显著（P = 0.0000183，费舍尔F检验）。有5个整合位点与脆性位点的位置不对应。13个整合位点与原癌基因定位于相同条带（37%），6个（17%）相距一个条带，在16个整合位点（46%）没有原癌基因定位（P = 0.00491）。18个病毒整合位点、脆性位点和原癌基因（51%）定位于相同条带或相距一个条带。病毒整合位点、脆性位点和原癌基因的这种聚集在统计学上非常显著（P = 0.0000118），并表明在人类基因组中存在非随机的病毒整合。
