Human and Animal Physiology, Wageningen University, De Elst 1, 6708 WD Wageningen, PO Box 338, 6700AH Wageningen, The Netherlands.
Exp Physiol. 2013 May;98(5):1053-62. doi: 10.1113/expphysiol.2012.069518. Epub 2012 Dec 13.
Understanding body weight regulation is essential to fight obesity. Mouse studies, using different types of diets, showed conflicting results in terms of body weight persistence after changing from an ad libitum high-fat diet to an ad libitum low-fat diet. In this study, we questioned specifically whether the energy content of the diet has a lasting effect on energy balance and body weight, using multiple switches and two purified diets with a different fat-to-sugar ratio, but otherwise identical ingredients. Young-adult obesity-prone male C57BL/6J mice were fed single or double switches of semi-purified diets with either 10 energy % (en%) fat (LF) or 40en% fat (HF), with starch replaced by fat, while protein content remained equal. After none, one or two dietary changes, energy metabolism was assessed at 5, 14 and 19 weeks. We observed no systematic continuous compensation in diet and energy intake when returning to LF after HF consumption. Body weight, white adipose tissue mass and histology, serum metabolic parameters, energy expenditure and substrate usage all significantly reflected the current diet intake, independent of dietary changes. This contrasts with studies that used diets with different ingredients and showed persistent effects of dietary history on body weight, suggesting diet-dependent metabolic set points. We conclude that body weight and metabolic parameters 'settle', based on current energetic input and output. This study also highlights the importance of considering the choice of diet in physiological and metabolic intervention studies.
了解体重调节对于对抗肥胖至关重要。使用不同类型饮食的小鼠研究在从高脂肪自由饮食转变为低脂肪自由饮食后体重持续存在方面显示出相互矛盾的结果。在这项研究中，我们特别质疑饮食的能量含量是否对能量平衡和体重产生持久影响，使用了多种切换和两种具有不同脂肪与糖比例但其他成分相同的纯化饮食。易肥胖的年轻成年雄性 C57BL/6J 小鼠喂食单一或双重切换半纯化饮食，其中 10 能量%（en%）脂肪（LF）或 40en%脂肪（HF），用脂肪代替淀粉，而蛋白质含量保持不变。在没有、一次或两次饮食改变后，在 5、14 和 19 周时评估能量代谢。我们观察到在从 HF 消耗后返回 LF 时，饮食和能量摄入没有系统的连续补偿。体重、白色脂肪组织质量和组织学、血清代谢参数、能量消耗和底物利用都显著反映了当前的饮食摄入，与饮食变化无关。这与使用不同成分饮食的研究形成对比，这些研究显示饮食史对体重有持续影响，表明饮食依赖代谢设定点。我们得出结论，体重和代谢参数“稳定”，基于当前的能量输入和输出。这项研究还强调了在生理和代谢干预研究中考虑饮食选择的重要性。
