Liang Yu-Jie, Wang Hui-Ping, Long Ding-Xin, Wu Yi-Jun
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
Bioanalysis. 2012 Dec;4(24):2897-907. doi: 10.4155/bio.12.277.
NMR combined with pattern recognition was recently introduced as a new technique for rapid xenobiotic toxicity evaluation. In this article, metabolic changes in the biofluid of rats after 90-day oral treatment with propoxur, permethrin and a combination of these two pesticides were investigated.
Propoxur dosing induced increased urinary taurine, creatinine and glucose, whereas urinary lactate and acetate were increased in the highest permethrin dose group. Urinary acetate, alanine, lactate and trimethylamine levels were increased in the mixture group, accompanied by decreased urinary tricarboxylic acid cycle intermediates. In addition, the highest dose of the mixture displayed raised 3-D-hydroxybutyrate, acetate and lactate levels in the serum sample.
Chronic exposure to a combination of propoxur and permethrin may induce hepatotoxicity and nephrotoxicity. An increase in acetate, alanine and formate in the urine could be a potentially sensitive biomarker of the chronic, combined effects of permethrin and propoxur.
核磁共振（NMR）结合模式识别技术最近作为一种快速评估外源化合物毒性的新技术被引入。本文研究了大鼠经口给予残杀威、氯菊酯以及这两种农药混合物90天后生物流体中的代谢变化。
给予残杀威导致尿中牛磺酸、肌酐和葡萄糖增加，而在氯菊酯最高剂量组中尿中乳酸和乙酸增加。混合物组中尿乙酸、丙氨酸、乳酸和三甲胺水平增加，同时尿三羧酸循环中间产物减少。此外，混合物最高剂量组血清样本中3 - D - 羟基丁酸、乙酸和乳酸水平升高。
长期接触残杀威和氯菊酯的混合物可能会诱发肝毒性和肾毒性。尿中乙酸、丙氨酸和甲酸的增加可能是氯菊酯和残杀威联合慢性作用的潜在敏感生物标志物。
