Wang Pan, Xu Ming-Yuan, Liang Yu-Jie, Wang Hui-Ping, Sun Ying-Jian, Long Ding-Xin, Wu Yi-Jun
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China.
Laboratory of Molecular Toxicology, State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, PR China; Department of Veterinary Medicine and Animal Science, Beijing University of Agriculture, Beijing 102206, PR China.
Chem Biol Interact. 2017 Jun 25;272:21-27. doi: 10.1016/j.cbi.2017.04.023. Epub 2017 Apr 26.
Carbamates and pyrethroids are widely used pesticides. However, their joint toxicity at low doses with long-term exposure remains unknown. Therefore, we investigated the subchronic joint hepatotoxicity of the two representative pesticides within these two classes, i.e., propoxur (PR) and permethrin (PE) in rats. The male Wistar rats were orally treated with three different doses of PR, PE and their mixtures for 90 consecutive days. Liver weight, serum clinical chemistry parameters and histopathological changes were measured to access the hepatotoxicity. In addition, oxidative stress markers in liver were measured using biochemical assays. The results showed that PR reduced liver weight and lead to prominent liver histological changes. Moreover, PR dose-dependently induced lipid peroxidation and reduced superoxide dismutase activity. In contrast, PE induced a relatively mild hepatotoxicity. Intriguingly, the mixture of PR and PE did not reduce liver weight or increase serum aspartate transaminase activity. In addition, the mixture did not reduce the antioxidant enzyme activity as PR did. Thus, these results showed that PR induced prominent hepatotoxicity with subchronic exposure, and there is a potential antagonistic interaction between PR and PE on the oxidative damage in liver of rats.
氨基甲酸盐类和拟除虫菊酯类是广泛使用的杀虫剂。然而，它们在低剂量长期暴露下的联合毒性仍不清楚。因此，我们研究了这两类中两种代表性杀虫剂，即残杀威（PR）和氯菊酯（PE）对大鼠的亚慢性联合肝毒性。雄性Wistar大鼠连续90天口服三种不同剂量的PR、PE及其混合物。测量肝脏重量、血清临床化学参数和组织病理学变化以评估肝毒性。此外，使用生化分析方法测量肝脏中的氧化应激标志物。结果表明，PR降低了肝脏重量并导致明显的肝脏组织学变化。此外，PR剂量依赖性地诱导脂质过氧化并降低超氧化物歧化酶活性。相比之下，PE诱导的肝毒性相对较轻。有趣的是，PR和PE的混合物并没有降低肝脏重量或增加血清天冬氨酸转氨酶活性。此外，该混合物没有像PR那样降低抗氧化酶活性。因此，这些结果表明，亚慢性暴露时PR会诱导明显的肝毒性，并且PR和PE在大鼠肝脏氧化损伤方面存在潜在的拮抗相互作用。
