Department of Internal Medicine, Chungnam National University School of Medicine, Daejeon, Korea.
Int J Rheum Dis. 2012 Dec;15(6):531-7. doi: 10.1111/j.1756-185X.2012.01813.x. Epub 2012 Sep 7.
There is growing evidence that two recently recognized, unique subsets of CD4(+) T-cells, T-helper 17 cells (Th17) and CD4(+) CD25(+) regulatory T-cells (Treg), may play important roles in the pathogenesis of psoriasis. This study sought to investigate the relationship between Th17 cells and psoriasis or psoriatic arthritis (PsA).
Peripheral blood CD4(+) T-cells were isolated from 47 patients with psoriasis and 47 controls, and were cultured under various stimulatory conditions. The Th17 cells and regulatory Treg cells were detected by flow cytometry. Plasma interleukin (IL)-17 was measured by enzyme-linked immunosorbent assay.
The proportion of Th17 and Treg cells from peripheral blood mononuclear cells showed no difference between patients with psoriasis and controls. However, psoriasis and PsA patients demonstrated a higher proportion of induced Th17 cells, which was correlated with psoriasis area severity index score. The level of plasma IL-17A in psoriasis was higher than that in controls.
Th17 cells may play important roles in the pathogenesis of psoriasis and psoriatic arthritis.
越来越多的证据表明，两种最近被认识的、独特的 CD4(+) T 细胞亚群，辅助性 T 细胞 17 细胞（Th17）和 CD4(+) CD25(+)调节性 T 细胞（Treg），可能在银屑病的发病机制中发挥重要作用。本研究旨在探讨 Th17 细胞与银屑病或银屑病关节炎（PsA）之间的关系。
从 47 例银屑病患者和 47 例对照者外周血单个核细胞中分离 CD4(+) T 细胞，并在不同刺激条件下进行培养。采用流式细胞术检测 Th17 细胞和调节性 Treg 细胞。采用酶联免疫吸附试验检测血浆白细胞介素（IL）-17。
银屑病患者和对照组外周血单个核细胞中 Th17 和 Treg 细胞的比例无差异。然而，银屑病和 PsA 患者诱导的 Th17 细胞比例较高，且与银屑病面积严重指数评分相关。银屑病患者血浆 IL-17A 水平高于对照组。
Th17 细胞可能在银屑病和银屑病关节炎的发病机制中发挥重要作用。
