Radford D R, Sweet S P, Challacombe S J, Walter J D
Department of Prosthetic Dentistry, UMDS, Guy's and St Thomas's Medical and Dental School, University of London, UK.
J Dent. 1998 Sep;26(7):577-83. doi: 10.1016/s0300-5712(97)00034-1.
To assess the in vitro adherence of Candida albicans to heat-cured hard and soft denture-base materials with varying surface roughness, and to observe the effect of a mixed salivary pellicle on candidal adhesion to these surfaces.
In vitro adhesion assays on heat-cured acrylic resin (Trevalon), Molloplast B and Novus using the type strain of C. albicans (NCPF 3153A). Surfaces for the assays were prepared using clinically appropriate rotary instruments. Unstimulated, pooled and clarified whole saliva was used to assess its effect on adhesion.
Significantly greater adhesion of C. albicans to rough rather than smooth surfaces was found (P < 0.001), as well as increased adhesion to the machined soft lining materials compared with acrylic. Pre-coating denture-base materials with saliva reduced candidal adhesion on all materials.
Rough surfaces on denture-base materials promote the adhesion of C. albicans in vitro. However, saliva reduces adhesion of C. albicans and thus diminishes the effect of surface roughness and free surface energy differences between materials.
评估白色念珠菌在体外对具有不同表面粗糙度的热固化硬质和软质义齿基托材料的黏附情况，并观察混合唾液薄膜对念珠菌在这些表面黏附的影响。
使用白色念珠菌标准菌株（NCPF 3153A）对热固化丙烯酸树脂（Trevalon）、Molloplast B和Novus进行体外黏附试验。试验表面使用临床适用的旋转器械制备。使用未刺激的、汇集并澄清的全唾液来评估其对黏附的影响。
发现白色念珠菌在粗糙表面而非光滑表面的黏附显著增加（P < 0.001），并且与丙烯酸树脂相比，其在加工后的软衬材料上的黏附也增加。用唾液预涂义齿基托材料可降低念珠菌在所有材料上的黏附。
义齿基托材料的粗糙表面在体外促进白色念珠菌的黏附。然而，唾液可降低白色念珠菌的黏附，从而减弱表面粗糙度和材料间自由表面能差异的影响。
