Institute for Biological Research 'Siniša Stanković', University of Belgrade, Boulevard Despota Stefana 142, 11060 Belgrade, Serbia.
Chem Biodivers. 2012 Dec;9(12):2761-74. doi: 10.1002/cbdv.201200051.
This is the first report on the composition and variability of the needle-wax n-alkanes in natural populations of Pinus nigra in Serbia. Samples of 195 trees from seven populations belonging to several infraspecific taxa (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica) were analyzed. In general, the size of the n-alkanes ranged from C(16) to C(33) , with the exception of ssp. nigra, for which it ranged from C(18) to C(33) . The most abundant were C(23) -, C(25) -, C(27) -, and C(29) -alkanes. The needle waxes of Populations I-III and V were characterized by a higher content of C(23) -, C(25) -, and C(27) -alkanes and a lower content of C(24) -, C(26) -, C(28) -, and C(30) -alkanes, compared to the other populations, and the trees of these populations could be assigned to ssp. nigra. The samples of Population VI were characterized by higher amounts of C(22) -, C(24) -, C(30) -, and C(32) -alkanes and lower amounts of C(25) - and C(27) -alkanes, and the trees could be considered as ssp. pallasiana. The samples of Population VII, consisting of trees belonging to var. banatica, were richer in C(29) -, C(31) -, and C(33) -alkanes. The wax compositions of Populations IV and V, both composed of trees previously determined as P. nigra var. gocensis, showed a tendency of splitting. Indeed, the alkane composition of Population IV was closer to that of ssp. pallasiana pines, while that of Population V was more similar to that of ssp. nigra pines. From the results presented here, it is obvious that in the central part of the Balkan Peninsula, significant diversification and differentiation of the populations of black pine exists, and these populations could be defined as different intraspecific taxa. Our results also indicate the validity of n-alkanes as chemotaxonomic characters within this aggregate.
这是关于塞尔维亚黑松自然种群针蜡正构烷烃组成和变异性的首份报告。对来自 7 个种群的 195 棵树的样本进行了分析，这些种群隶属于几个种下分类群（ssp. nigra、var. gocensis、ssp. pallasiana 和 var. banatica）。通常，正构烷烃的大小范围为 C(16) 到 C(33)，但 ssp. nigra 的范围为 C(18) 到 C(33)。最丰富的是 C(23)、C(25)、C(27) 和 C(29)-烷烃。种群 I-III 和 V 的针蜡蜡以更高含量的 C(23)、C(25)和 C(27)-烷烃和更低含量的 C(24)、C(26)、C(28)和 C(30)-烷烃为特征，与其他种群相比，这些种群的树木可被归类为 ssp. nigra。种群 VI 的样本的特征是 C(22)、C(24)、C(30)和 C(32)-烷烃的含量较高，C(25)和 C(27)-烷烃的含量较低，这些树木可以被认为是 ssp. pallasiana。种群 VII 的样本由属于 var. banatica 的树木组成，富含 C(29)、C(31)和 C(33)-烷烃。种群 IV 和 V 的蜡组成，均由先前确定为 P. nigra var. gocensis 的树木组成，表现出分裂的趋势。实际上，种群 IV 的烷烃组成更接近 ssp. pallasiana 松树，而种群 V 的烷烃组成则更类似于 ssp. nigra 松树。从这里呈现的结果可以明显看出，在巴尔干半岛的中心部分，黑松种群存在显著的多样化和分化，这些种群可以被定义为不同的种下分类群。我们的结果还表明，正构烷烃作为该聚合体内部的化学分类特征是有效的。
