University of Niš, Faculty of Sciences and Mathematics, Višegradska 33, RS-18000 Niš, Serbia.
Chem Biodivers. 2013 Aug;10(8):1507-20. doi: 10.1002/cbdv.201200416.
The essential-oil variability in seven native populations belonging to different infraspecific taxa of Pinus nigra (ssp. nigra, var. gocensis, ssp. pallasiana, and var. banatica) growing wild in Serbia was analyzed. In the needles of 195 trees from seven populations, 58 essential-oil components were identified. The major components were α-pinene (43.6%) and germacrene D (29.8%), comprising together 73.4% of the total oil composition. Based on the average chemical profile of the main terpene components (with contents >5%), the studied populations were found to be the most similar to populations from central Italy and Greece (ssp. nigra). Cluster analysis showed the division of the populations into three principal groups: the first group consisted of Populations I, II, III, IV, and V (considered as ssp. nigra group), the second of Population VI (ssp. pallasiana group), and the third of Population VII, which had the most distinct oil composition (ssp. banatica group). The taxonomic implications of the essential-oil profiles of the investigated taxa of this very complex species are discussed.
分析了生长在塞尔维亚的七个不同种下分类群（黑松亚种、戈岑亚种、派勒斯亚种和巴塔尼卡亚种）的七个本地种群的 7 种精油的变异性。在来自七个种群的 195 棵树的针叶中，鉴定出了 58 种精油成分。主要成分是α-蒎烯（43.6%）和反式-杜松烯 D（29.8%），占总油组成的 73.4%。基于主要萜烯成分（含量>5%）的平均化学特征，研究的种群与来自意大利中部和希腊的种群（黑松亚种）最为相似。聚类分析显示，种群分为三个主要组：第一组由种群 I、II、III、IV 和 V 组成（被认为是黑松亚种组），第二组由种群 VI 组成（派勒斯亚种组），第三组由种群 VII 组成，其精油组成最为独特（巴塔尼卡亚种组）。讨论了调查的非常复杂物种的类群的精油特征的分类学意义。
