Wong V G, Green W R, Liu Y P, Marsden E R
Trans Am Ophthalmol Soc. 1979;77:63-79.
Transplantable Brown-Pearce carcinoma was adapted successfully in the rabbit anterior chamber. Regression of tumor growth was attained on tri-weekly perfusion of the AC with 10 micromolar of methotrexate. Tumor cyclic nucleotide phosphodiesterase (PDE) and protein activator were found to be markedly depressed during the course of chemotherapy and the PDE cAMP/cGMP ratio was similarly altered. Corroborative light and electron-microscopic studies showed specific alterations of intracellular organelles in relation to MTX and tumor cell death. These findings suggest that metabolic pathways of cyclic nucleotides are important biochemical modulators of neoplastic cells. The method of intraocular perfusion precludes systemic toxic effects and avoids compromising the animals' immunocompetence.
可移植性布朗-皮尔斯癌在兔眼前房成功适应。通过每周三次用10微摩尔甲氨蝶呤灌注前房，肿瘤生长得到了消退。在化疗过程中发现肿瘤环核苷酸磷酸二酯酶（PDE）和蛋白激活剂明显降低，并且PDE的cAMP/cGMP比值也有类似改变。对照的光镜和电镜研究显示细胞内细胞器与甲氨蝶呤和肿瘤细胞死亡相关的特异性改变。这些发现表明环核苷酸的代谢途径是肿瘤细胞重要的生化调节剂。眼内灌注方法避免了全身毒性作用，并避免损害动物的免疫能力。
