Tsvetkov P, Petkova K, Bachiĭski L, Kharalambiev Kh E
Vet Med Nauki. 1979;16(5):3-9.
Bivalent inactivated vaccine against mucous disease (MD) and infectious rhinotracheitis (IR) in cattle was produced from cell cultural MD and IR virus suspensions. The vaccine was concentrated on aluminium hydroxide, inactivated by ethanol and is without residual virus. Saponine in final 1:1500 dilution is added as supplementary adjuvant. Immunogeneity of the vaccine was tested on 10-month-old calves, which had shown full resistance against experimental infection with virulent strains of both viruses. Testing on calves for harmlessness by use of a five-fold higher vaccine dose indicated complete tolerance of the vaccine. The prophylactic effect of the vaccine applied in practical work to directly threatened with immediate MD and IR infection cows, including pregnant ones, consisted in reduced number of cases of abortion, of inborn malformations, in lower neonatal calf death-rate, etc. No disturbances were observed following two-fold vaccination of the animals, a fact proving its harmlessness. The positive results of the studied vaccine allow its further application in the combined prophylaxis of MD and IR in calf fattening and breeding complexes.
牛黏液病（MD）和传染性鼻气管炎（IR）二价灭活疫苗由细胞培养的MD和IR病毒悬液制备而成。该疫苗用氢氧化铝浓缩，经乙醇灭活且无残余病毒。最终稀释度为1:1500时添加皂苷作为辅助佐剂。疫苗免疫原性在10月龄犊牛上进行测试，这些犊牛对两种病毒的强毒株实验感染均表现出完全抗性。使用五倍高剂量疫苗对犊牛进行无害性测试表明疫苗完全耐受。该疫苗在实际工作中应用于直接受到MD和IR感染威胁的奶牛（包括怀孕奶牛），其预防效果包括减少流产病例数、先天性畸形数、降低新生犊牛死亡率等。对动物进行两倍剂量接种后未观察到任何干扰，这一事实证明了其无害性。所研究疫苗的阳性结果使其可进一步应用于犊牛育肥和繁殖综合体中MD和IR的联合预防。
