Departamento de Tocoginecologia, Faculdade de Ciências Médicas, Campinas University, Campinas, São Paulo, Brazil.
Eur J Obstet Gynecol Reprod Biol. 2013 Mar;167(1):96-9. doi: 10.1016/j.ejogrb.2012.11.008. Epub 2012 Dec 21.
Reduced CD16 expression is associated with neutrophil apoptosis. This study aimed to compare CD16 expression on neutrophils in the vagina from women with normal bacterial flora and with vaginitis.
Vaginal lavages were sampled from volunteers diagnosed with bacterial vaginosis (BV, n=34), vulvovaginal candidiasis (VC, n=43), BV plus VC (BV+VC, n=14), and normal flora (NF, n=51). Neutrophils were identified by expression of CD15, CD16 and CD24 surface markers as assessed by flow cytometry.
CD16 expression was elevated in neutrophils from women with vaginitis (BV p<0.0001; VC p=0.01; BV+VC p=0.0027) as compared to women with NF.
The reduction in CD16 down-regulation is consistent with prolonged neutrophil viability and activity in the vagina of women with vaginitis. This may contribute to greater microbial clearance and, conversely, with inflammation-associated pathology.
CD16 表达减少与中性粒细胞凋亡有关。本研究旨在比较正常菌群和阴道炎患者阴道中性粒细胞上的 CD16 表达。
从被诊断为细菌性阴道病（BV，n=34）、外阴阴道念珠菌病（VC，n=43）、BV+VC（BV+VC，n=14）和正常菌群（NF，n=51）的志愿者中采集阴道灌洗液。通过流式细胞术评估中性粒细胞表面标志物 CD15、CD16 和 CD24 的表达来鉴定中性粒细胞。
与 NF 女性相比，阴道炎患者（BV p<0.0001；VC p=0.01；BV+VC p=0.0027）阴道中性粒细胞 CD16 表达升高。
CD16 下调减少与阴道炎患者阴道中性粒细胞活力和活性延长一致。这可能有助于更好地清除微生物，相反，与炎症相关的病理学有关。
