Schuster W, Unseld M, Wissinger B, Brennicke A
Institut für Genbiologische Forschung, Berlin, FRG.
Nucleic Acids Res. 1990 Jan 25;18(2):229-33. doi: 10.1093/nar/18.2.229.
The gene encoding ribosomal protein S14 (rps14) in Oenothera mitochondria is located upstream of the cytochrome b gene (cob). Sequence analysis of independently derived cDNA clones covering the entire rps14 coding region shows two nucleotides edited from the genomic DNA to the mRNA derived sequences by C to U modifications. A third editing event occurs four nucleotides upstream of the AUG initiation codon and improves a potential ribosome binding site. A CGG codon specifying arginine in a position conserved in evolution between chloroplasts and E. coli as a UGG tryptophan codon is not edited in any of the cDNAs analysed. An inverted repeat 3' of an unidentified open reading frame is located upstream of the rps14 gene. The inverted repeat sequence is highly conserved at analogous regions in other Oenothera mitochondrial loci.
月见草线粒体中编码核糖体蛋白S14（rps14）的基因位于细胞色素b基因（cob）的上游。对覆盖整个rps14编码区的独立获得的cDNA克隆进行序列分析表明，通过C到U的修饰，基因组DNA中的两个核苷酸在mRNA衍生序列中被编辑。第三个编辑事件发生在AUG起始密码子上游四个核苷酸处，并改善了一个潜在的核糖体结合位点。在叶绿体和大肠杆菌之间进化上保守的位置上指定精氨酸的CGG密码子在分析的任何cDNA中都未被编辑。一个未鉴定的开放阅读框的反向重复序列位于rps14基因的上游。该反向重复序列在其他月见草线粒体基因座的类似区域高度保守。
