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用于研究稳定mRNA分子衰变的诱导型启动子系统的表征

Characterization of an inducible promoter system to investigate decay of stable mRNA molecules.

作者信息

Helms S R, Rottman F M

机构信息

Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, OH.

出版信息

Nucleic Acids Res. 1990 Jan 25;18(2):255-9. doi: 10.1093/nar/18.2.255.

DOI:10.1093/nar/18.2.255
PMID:2326163
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC330261/
Abstract

We have developed a system in which the decay of stable mRNAs can be studied without the use of inhibitors of transcription. The Drosophila hsp70 heat shock promoter linked to the bovine growth hormone (BGH) gene was used to establish stable cell lines in which the BGH gene is transcribed in a conditional manner. The BGH mRNA is synthesized only after induction at 43 degrees C. Following a brief period of re-equilibration at 37 degrees C during which transcription of the heat shock-driven gene ceases, the stable BGH mRNA decays with typical first-order kinetics. Hence, the decay of the mRNA can be studied without assumptions regarding radioactive labeling of precursor pools or transcriptional inhibitors. The system is applicable to any stable mRNA.

摘要

我们开发了一种系统，利用该系统可以在不使用转录抑制剂的情况下研究稳定mRNA的降解过程。将与牛生长激素（BGH）基因相连的果蝇hsp70热休克启动子用于建立稳定的细胞系，其中BGH基因以条件性方式转录。BGH mRNA仅在43℃诱导后才合成。在37℃短暂重新平衡期间，热休克驱动基因的转录停止，稳定的BGH mRNA以典型的一级动力学衰减。因此，可以在不假设前体池放射性标记或转录抑制剂的情况下研究mRNA的降解。该系统适用于任何稳定的mRNA。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f9b/330261/a88dc428fc66/nar00186-0043-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f9b/330261/d15dba70f698/nar00186-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f9b/330261/8d83305c4e18/nar00186-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f9b/330261/eed10479d24b/nar00186-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f9b/330261/a88dc428fc66/nar00186-0043-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f9b/330261/d15dba70f698/nar00186-0042-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f9b/330261/8d83305c4e18/nar00186-0043-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f9b/330261/eed10479d24b/nar00186-0043-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3f9b/330261/a88dc428fc66/nar00186-0043-c.jpg

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本文引用的文献

1
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Proc Natl Acad Sci U S A. 1982 May;79(10):3167-70. doi: 10.1073/pnas.79.10.3167.
2
Sequence of three copies of the gene for the major Drosophila heat shock induced protein and their flanking regions.果蝇主要热休克诱导蛋白基因的三个拷贝序列及其侧翼区域。
Cell. 1980 Oct;21(3):669-79. doi: 10.1016/0092-8674(80)90430-4.
3
Cloning and nucleotide sequencing of the bovine growth hormone gene.
AUUUA不足以促进mRNA的多聚腺苷酸化缩短和降解：富含AU元件中的功能序列可能是UUAUUUA（U/A）（U/A）。
Mol Cell Biol. 1994 Dec;14(12):7984-95. doi: 10.1128/mcb.14.12.7984-7995.1994.
4
Dexamethasone inducible gene expression optimised by glucocorticoid antagonists.通过糖皮质激素拮抗剂优化地塞米松诱导的基因表达。
Nucleic Acids Res. 1995 Jun 25;23(12):2342-3. doi: 10.1093/nar/23.12.2342.
5
mRNA stability in mammalian cells.哺乳动物细胞中的信使核糖核酸稳定性
Microbiol Rev. 1995 Sep;59(3):423-50. doi: 10.1128/mr.59.3.423-450.1995.
6
Newly synthesized RNA: simultaneous measurement in intact cells of transcription rates and RNA stability of insulin-like growth factor I, actin, and albumin in growth hormone-stimulated hepatocytes.
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Nucleic Acids Res. 1982 Nov 25;10(22):7197-210. doi: 10.1093/nar/10.22.7197.
4
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5
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6
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7
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8
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10
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J Mol Biol. 1973 Aug 5;78(2):321-34. doi: 10.1016/0022-2836(73)90119-8.