Dreano M, Brochot J, Myers A, Cheng-Meyer C, Rungger D, Voellmy R, Bromley P
Gene. 1986;49(1):1-8. doi: 10.1016/0378-1119(86)90380-x.
Plasmids have been constructed in which promoters of 70-kDa heat-shock protein genes (hsp70) of human and Drosophila origin were linked to three different eukaryotic genes encoding human growth hormone (hGH), chicken lysozyme (cL) and a human influenza haemagglutinin (HA). Following transfection into widely divergent eukaryotic cells, the hybrid genes direct the transient, heat-regulated synthesis of the three proteins. hGH and cL are secreted into the medium. A human hsp70-hGH construct was used to establish stable mouse fibroblast lines that are capable of producing and secreting hGH at high levels following heat induction: hGH is secreted at a 500-1200-fold higher rate by heat-treated than by untreated cells.
已构建出质粒，其中人类和果蝇来源的70-kDa热休克蛋白基因（hsp70）的启动子与编码人类生长激素（hGH）、鸡溶菌酶（cL）和人类流感血凝素（HA）的三种不同真核基因相连。转染到广泛不同的真核细胞后，杂种基因指导这三种蛋白质的瞬时、热调节合成。hGH和cL分泌到培养基中。一个人类hsp70-hGH构建体被用于建立稳定的小鼠成纤维细胞系，该细胞系在热诱导后能够高水平产生和分泌hGH：热处理细胞分泌hGH的速率比未处理细胞高500 - 1200倍。
